| Sonic hedgehog controls growth of external genitalia by regulating cell cycle kinetics. | |
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MedLine Citation:
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PMID: 20975695 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During embryonic development, cells are instructed which position to occupy, they interpret these cues as differentiation programmes, and expand these patterns by growth. Sonic hedgehog (Shh) specifies positional identity in many organs; however, its role in growth is not well understood. In this study, we show that inactivation of Shh in external genitalia extends the cell cycle from 8.5 to 14.4 h, and genital growth is reduced by ∼75%. Transient Shh signalling establishes pattern in the genital tubercle; however, transcriptional levels of G1 cell cycle regulators are reduced. Consequently, G1 length is extended, leading to fewer progenitor cells entering S-phase. Cell cycle genes responded similarly to Shh inactivation in genitalia and limbs, suggesting that Shh may regulate growth by similar mechanisms in different organ systems. The finding that Shh regulates cell number by controlling the length of specific cell cycle phases identifies a novel mechanism by which Shh elaborates pattern during appendage development. |
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Authors:
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Ashley W Seifert; Zhengui Zheng; Brandi K Ormerod; Martin J Cohn |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-06-01 |
Journal Detail:
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Title: Nature communications Volume: 1 ISSN: 2041-1723 ISO Abbreviation: Nat Commun Publication Date: 2010 |
Date Detail:
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Created Date: 2011-03-22 Completed Date: 2011-07-01 Revised Date: 2011-08-16 |
Medline Journal Info:
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Nlm Unique ID: 101528555 Medline TA: Nat Commun Country: England |
Other Details:
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Languages: eng Pagination: 23 Citation Subset: IM |
Affiliation:
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Department of Biology, University of Florida, Gainesville, FL 32611, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle / genetics, physiology* Extremities / embryology Female Genitalia / embryology*, metabolism* Hedgehog Proteins / genetics, metabolism* Immunohistochemistry In Situ Hybridization Mice Pregnancy Reverse Transcriptase Polymerase Chain Reaction |
| Grant Support | |
ID/Acronym/Agency:
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1R01 HD054554/HD/NICHD NIH HHS; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
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0/Hedgehog Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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