| Son is essential for nuclear speckle organization and cell cycle progression. | |
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MedLine Citation:
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PMID: 20053686 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Subnuclear organization and spatiotemporal regulation of pre-mRNA processing factors is essential for the production of mature protein-coding mRNAs. We have discovered that a large protein called Son has a novel role in maintaining proper nuclear organization of pre-mRNA processing factors in nuclear speckles. The primary sequence of Son contains a concentrated region of multiple unique tandem repeat motifs that may support a role for Son as a scaffolding protein for RNA processing factors in nuclear speckles. We used RNA interference (RNAi) approaches and high-resolution microscopy techniques to study the functions of Son in the context of intact cells. Although Son precisely colocalizes with pre-mRNA splicing factors in nuclear speckles, its depletion by RNAi leads to cell cycle arrest in metaphase and causes dramatic disorganization of small nuclear ribonuclear protein and serine-arginine rich protein splicing factors during interphase. Here, we propose that Son is essential for appropriate subnuclear organization of pre-mRNA splicing factors and for promoting normal cell cycle progression. |
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Authors:
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Alok Sharma; Hideaki Takata; Kei-ichi Shibahara; Athanasios Bubulya; Paula A Bubulya |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-01-06 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 21 ISSN: 1939-4586 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-12 Completed Date: 2010-07-19 Revised Date: 2010-09-28 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 650-63 Citation Subset: IM |
Affiliation:
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Biomedical Sciences Ph.D. Program, Department of Biological Sciences, Wright State University, Dayton, OH 45435, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Cell Cycle / physiology* Cell Nucleus / metabolism*, ultrastructure Cell Survival DNA-Binding Proteins / genetics, metabolism* Hela Cells Humans Molecular Sequence Data Neoplasm Proteins / genetics, metabolism Nuclear Proteins / genetics, metabolism RNA Interference RNA Precursors / genetics, metabolism RNA-Binding Proteins / genetics, metabolism Recombinant Fusion Proteins / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R15GM-084407/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CASC3 protein, human; 0/DNA-Binding Proteins; 0/Neoplasm Proteins; 0/Nuclear Proteins; 0/RNA Precursors; 0/RNA-Binding Proteins; 0/Recombinant Fusion Proteins; 0/SON protein, human; 170974-22-8/serine-arginine-rich splicing proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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