Document Detail


Some stereological correction formulae with particular applications in quantitative neurohistology.
MedLine Citation:
PMID:  1206428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present report furnishes explicit formulations for correcting the observed nucleolar and nuclear volume fractions of neurons sampled on the basis of the presence of nucleolar profiles when sectioned. Such samples, frequently chosen for quantitative studies in neurohistology, can result in the overestimation ov component volume fractions. The correction formulae are derived by considering theoretical spherical models exhibiting "nucleus-nucleolus" concentricity and eccentricity, and are tested by applying them to analogous neuron populations in rat spinal cord. It is concluded that, with certain reservations, the formulae can be usefully applied to data obtained from nucleolar-biased samples, thereby increasing the reliability of quantitative information gleaned from such samples.
Authors:
T M Mayhew; L M orive
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of the neurological sciences     Volume:  26     ISSN:  0022-510X     ISO Abbreviation:  J. Neurol. Sci.     Publication Date:  1975 Dec 
Date Detail:
Created Date:  1976-03-24     Completed Date:  1976-03-24     Revised Date:  2005-02-14    
Medline Journal Info:
Nlm Unique ID:  0375403     Medline TA:  J Neurol Sci     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  503-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleolus*
Cell Nucleus
Karyometry / methods*
Models, Biological
Nervous System / anatomy & histology*,  ultrastructure

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