Document Detail


Some properties of yeast mitochondria prepared by a rapid mechanical procedure.
MedLine Citation:
PMID:  788796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method is described for preparing yeast mitochondria rapidly (within one hour) by using the MSK Bronwill Cell homogenizer. Yeast mitochondria obtained by the method exhibit relatively good respiratory controls and ADP/O ratios. The method is convenient for small or large amounts of yeast cells (from 5 to a hundred grams, wet weight) and gave a yield of 3 to 5 mg protein/g wet weight of yeast mitochondria.
Authors:
H Chambon; P Labbe
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimie     Volume:  58     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  1976  
Date Detail:
Created Date:  1977-01-03     Completed Date:  1977-01-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  837-42     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Fractionation / methods
Cold Temperature
Cytochromes / analysis
Fungal Proteins / analysis
Mitochondria* / metabolism
Oxidative Phosphorylation
Oxygen Consumption
Polarography
Saccharomyces cerevisiae / ultrastructure*
Spectrophotometry
Chemical
Reg. No./Substance:
0/Cytochromes; 0/Fungal Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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