Document Detail


Some properties of flammutoxin from the edible mushroom Flammulina velutipes.
MedLine Citation:
PMID:  3433291     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A cytolytic toxin from the basidiocarps of the edible mushroom Flammulina velutipes was purified to homogeneity. The lysin, flammutoxin, is a single polypeptide chain of Mr 32,000 and pK about 5.4. It contains unusually large amounts of tryptophan, serine and glycine, and few or none of the sulfur-containing amino acids. Erythrocytes of rat, rabbit, guinea pig, man, mouse, cat and dog were sensitive to lysis, in that order, whereas erythrocytes of sheep, ox, goat, swine and horse were largely or completely resistant to lysis. The toxin appears not to be a phospholipase and it was not inhibitable by any of a variety of lipids. Hemolysis probably involves alteration of the erythrocyte membrane, with formation of submicroscopic ion channels, and it appears to be of the osmotic type. In some respects flammutoxin resembles phallolysin, a cytolytic toxin obtained from the mushroom Amanita phalloides.
Authors:
A W Bernheimer; J D Oppenheim
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  25     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  1987  
Date Detail:
Created Date:  1988-03-07     Completed Date:  1988-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1145-52     Citation Subset:  IM    
Affiliation:
Department of Microbiology, New York University School of Medicine, NY 10016.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Basidiomycota / analysis*
Calcium / pharmacology
Cats
Chromatography, Gel
Chromatography, Ion Exchange
Dogs
Erythrocytes / drug effects
Fungal Proteins / analysis*,  isolation & purification,  toxicity
Goats
Guinea Pigs
Hemolysis / drug effects
Horses
Humans
Isoelectric Focusing
Lipids / analysis
Molecular Weight
Mycotoxins / analysis*,  isolation & purification,  toxicity
Rabbits
Rats
Swine
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Fungal Proteins; 0/Lipids; 0/Mycotoxins; 55599-71-8/FTX protein, Flammulina velutipes; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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