Document Detail

Some observations in freeze-drying of recombinant bioluminescent Escherichia coli for toxicity monitoring.
MedLine Citation:
PMID:  11403844     Owner:  NLM     Status:  MEDLINE    
A recombinant bioluminescent bacteria, containing a fabA::luxCDABE fusion gene, has been used to characterize freeze-drying methods, which may be conveniently used as a tool for the development of a portable biosensor. Through residual water, viability, biosensing activity and scanning electron microscopy analyses, the characteristics that four cryoprotectants, trehalose, sucrose, sorbitol, and mannitol, conferred on freeze-dried samples were elucidated, including the morphology, water content and activity of the cells. It was found that trehalose showed the best freeze-drying efficiency among the tested cryoprotectants and it might have a specific capacity limitation in protection of the cells during the freeze step. Humidity might result in damage to the cells, according to the viability, when exposed to air during storage, while the water remaining post freeze-drying showed good correlation with damage to the freeze-dried cells when under air-tight storage conditions. The results with other recombinant bioluminescent bacteria indicated that these findings might be general features of the freeze-drying processes.
M B Gu; S H Choi; S W Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biotechnology     Volume:  88     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-06-13     Completed Date:  2001-09-13     Revised Date:  2007-01-23    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  95-105     Citation Subset:  IM    
Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology (K-JIST), 1 Oryong-dong, Puk-gu, 500-712, Kwangju, South Korea.
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MeSH Terms
Biosensing Techniques
Chemiluminescent Measurements*
Chlorophenols / toxicity
Cryoprotective Agents / pharmacology
Escherichia coli / drug effects,  genetics,  physiology*
Freeze Drying
Mannitol / pharmacology
Microbiological Techniques
Microscopy, Electron, Scanning
Sorbitol / pharmacology
Sucrose / pharmacology
Toxicity Tests / methods*
Trehalose / pharmacology
Reg. No./Substance:
0/Chlorophenols; 0/Cryoprotective Agents; 106-48-9/4-chlorophenol; 50-70-4/Sorbitol; 57-50-1/Sucrose; 69-65-8/Mannitol; 99-20-7/Trehalose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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