| Some observations on the carbohydrate composition of purified transferrin. | |
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MedLine Citation:
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PMID: 856748 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Five subfractions were isolated on DEAE-cellulose from samples of a commercially available human transferrin preparation and their carbohydrate composition was analysed. Hexosamine, galactose and total hexose were determined in four subfractions and sialic acid in all five. The data obtained indicate that the excess number of electrophoretic bands observed in transferrin from this source is due to the loss of carbohydrates which only affects sialic acid and none of the other sugar types. The availability of the penultimate galactose residues as the terminal residues in the subfractions deficient in sialic acid was also confirmed by a biological test utilizing the rat liver. The reason for the gradual loss of sialic acid from transferrin is unknown. Freezing-thawing and lyophilization did not detectably affect the sialic acid content of purified transferrin. However, free sialic acid did appear in some preparations on storage. It is concluded that similar changes in the carbohydrate composition of other plasma glycoproteins before, during, or after purification can be expected to exert an adverse effect on their usefulness as metabolic tracers. |
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Authors:
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K L Wong; E Regoeczi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of peptide and protein research Volume: 9 ISSN: 0367-8377 ISO Abbreviation: Int. J. Pept. Protein Res. Publication Date: 1977 |
Date Detail:
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Created Date: 1977-06-22 Completed Date: 1977-06-22 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0330420 Medline TA: Int J Pept Protein Res Country: DENMARK |
Other Details:
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Languages: eng Pagination: 241-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Availability Carbohydrates / analysis* Chromatography, DEAE-Cellulose Electrophoresis, Polyacrylamide Gel Freeze Drying Freezing Galactose / analysis Hexosamines / analysis Hexoses / analysis Humans Liver / metabolism Methods Rats Sialic Acids / analysis Transferrin / analysis*, isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Hexosamines; 0/Hexoses; 0/Sialic Acids; 11096-37-0/Transferrin; 26566-61-0/Galactose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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