Document Detail


Some observations on the carbohydrate composition of purified transferrin.
MedLine Citation:
PMID:  856748     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Five subfractions were isolated on DEAE-cellulose from samples of a commercially available human transferrin preparation and their carbohydrate composition was analysed. Hexosamine, galactose and total hexose were determined in four subfractions and sialic acid in all five. The data obtained indicate that the excess number of electrophoretic bands observed in transferrin from this source is due to the loss of carbohydrates which only affects sialic acid and none of the other sugar types. The availability of the penultimate galactose residues as the terminal residues in the subfractions deficient in sialic acid was also confirmed by a biological test utilizing the rat liver. The reason for the gradual loss of sialic acid from transferrin is unknown. Freezing-thawing and lyophilization did not detectably affect the sialic acid content of purified transferrin. However, free sialic acid did appear in some preparations on storage. It is concluded that similar changes in the carbohydrate composition of other plasma glycoproteins before, during, or after purification can be expected to exert an adverse effect on their usefulness as metabolic tracers.
Authors:
K L Wong; E Regoeczi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of peptide and protein research     Volume:  9     ISSN:  0367-8377     ISO Abbreviation:  Int. J. Pept. Protein Res.     Publication Date:  1977  
Date Detail:
Created Date:  1977-06-22     Completed Date:  1977-06-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0330420     Medline TA:  Int J Pept Protein Res     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  241-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Availability
Carbohydrates / analysis*
Chromatography, DEAE-Cellulose
Electrophoresis, Polyacrylamide Gel
Freeze Drying
Freezing
Galactose / analysis
Hexosamines / analysis
Hexoses / analysis
Humans
Liver / metabolism
Methods
Rats
Sialic Acids / analysis
Transferrin / analysis*,  isolation & purification
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Hexosamines; 0/Hexoses; 0/Sialic Acids; 11096-37-0/Transferrin; 26566-61-0/Galactose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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