Document Detail

Some electrical and pharmacological properties of gap junctions between adult ventricular myocytes.
MedLine Citation:
PMID:  3933364     Owner:  NLM     Status:  MEDLINE    
Ventricular myocytes were isolated from adult rat hearts using the technique of Wittenberg and Robinson (Cell Tissue Res. 216: 231-251, 1981). These cells exhibited morphology, input resistance, time constant, and excitability expected for cells in intact cardiac tissue. Pairs of these cells were electronically coupled, and junctional conductance was unaffected by transjunctional potential or hyperpolarization of both cells. Brief exposure of cell pairs to medium equilibrated with 100% CO2 or containing 0.1 mM octanol quickly and reversibly decreased junctional conductance. We conclude that gap junctions between pairs of ventricular myocytes possess physiological properties like those of junctions in many other tissues. This preparation will be useful in evaluating drug action on junctional communication in heart.
R L White; D C Spray; A C Campos de Carvalho; B A Wittenberg; M V Bennett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  249     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1985 Nov 
Date Detail:
Created Date:  1985-12-12     Completed Date:  1985-12-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C447-55     Citation Subset:  IM    
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MeSH Terms
Carbon Dioxide / pharmacology
Electric Wiring
Electrophysiology / instrumentation
Intercellular Junctions / drug effects,  physiology*
Myocardium / cytology*
Octanols / pharmacology
Rats, Inbred Strains
Grant Support
Reg. No./Substance:
0/Octanols; 124-38-9/Carbon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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