Document Detail

Some effects of acute and chronic dosing with aflatoxin B1 on rat liver nuclei.
MedLine Citation:
PMID:  178433     Owner:  NLM     Status:  MEDLINE    
A study has been made of changes occurring in rat liver nuclei as a result of the chronic and acute administration of aflatoxin B1. This has involved resolution of the nuclei into populations of differing ploidy by means of centrifugation in a zonal rotor. Chronic feeding of the toxin to weanling rats prevents the development of the predominant tetraploid hepatocyte nuclear population, which normally takes place during maturation. Increased populations of diploid and octaploid hepatocyte nuclei are observed. Chronic feeding of the toxin to adult animals also causes a reduction in the tetraploid population already established and, again, leads to increased numbers of diploid and octaploid nuclei. Once an abnormal nuclear population has been established by feeding the toxin, it persists until the development of hepatocarcinoma (if a 6-week carcinogenic feeding regimen has been used). The hepatomas have diploid nuclei. Labeling hepatic RNA and DNA in vivo has indicated that feeding the toxin causes a reversion to the immature distribution of DNA synthesis among the nuclear population, with little effect on the pattern of distribution of RNA synthesis. Acute administration of aflatoxin to adult rats also causes a reduction in the size of the tetraploid population, with increased proportions of diploid, octaploid, and higherploidy nuclei. These results are discussed in terms of a dual action of the toxin, antimitotic and necrogenic, and the possible relationship of these to the carcinogenic process.
G E Neal; D J Judah; W H Butler
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer research     Volume:  36     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1976 May 
Date Detail:
Created Date:  1976-08-02     Completed Date:  1976-08-02     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1771-8     Citation Subset:  IM    
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MeSH Terms
Aflatoxins / administration & dosage,  pharmacology*
Carcinoma, Hepatocellular / chemically induced,  pathology
Cell Nucleus / analysis,  drug effects
Centrifugation, Density Gradient / methods
DNA / analysis,  biosynthesis
Liver / cytology,  drug effects*,  pathology
Liver Neoplasms / chemically induced,  pathology
Neoplasms, Experimental / chemically induced,  pathology
Proteins / analysis
RNA / biosynthesis
Reg. No./Substance:
0/Aflatoxins; 0/Proteins; 63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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