Document Detail

Some difficulties in research into cell motile activity under isotropic conditions.
MedLine Citation:
PMID:  17687929     Owner:  NLM     Status:  MEDLINE    
Movement of Dictyostelium discoideum amoebae under isotropic and anisotropic conditions was recorded and analysed with computer-aided methods and the results are presented in various manners as described in the subject literature. Cell movement under isotropic conditions showed great diversity. Some cells moved almost in a straight path whereas others in close proximity turned around with little net translocation. When cell movement under isotropic conditions was observed, no direct correlation was found between the total length of cell trajectories and the length of final displacements of the cells. It was necessary to present the results in the form of histograms, circular diagrams of cell trajectories or in scatter correlation diagrams showing the motile behaviour of many individual cells. These methods of presentation are more informative than methods which present only average values, the "representative" behaviour of single cells, or start and end points of cell tracks. The latter methods can only illustrate but do not document the results of experiments. The use of statistical methods appears necessary in cases when it is difficult to monitor the same cells before and during experimental treatment. However, when cell movement under anisotropic conditions becomes oriented and ordered as during tactic cell movements, then the diversity in cell behaviour decreases and methods based on estimation of starting and end points of cell positions appear more credible.
Agnieszka Waligórska; Magdalena Wianecka-Skoczeń; Paweł Nowak; Włodzimierz Korohoda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Folia biologica     Volume:  55     ISSN:  0015-5497     ISO Abbreviation:  Folia Biol. (Krakow)     Publication Date:  2007  
Date Detail:
Created Date:  2007-08-10     Completed Date:  2007-09-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984758R     Medline TA:  Folia Biol (Krakow)     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  9-16     Citation Subset:  IM    
Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
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MeSH Terms
Cell Movement / physiology*
Chemotactic Factors / physiology
Dictyostelium / physiology*
Folic Acid / physiology
Image Processing, Computer-Assisted
Reg. No./Substance:
0/Chemotactic Factors; 59-30-3/Folic Acid

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