| Somatostatin precursor in the rat striatum: changes after local injection of kainic acid. | |
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MedLine Citation:
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PMID: 2864394 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The molecular forms of somatostatin contained in the rat striatum were separated by size-exclusion HPLC. Three major peaks of somatostatin-like immunoreactivity (SLI) were resolved. Two peaks cochromatographed with synthetic somatostatin-14 (SS-14) and somatostatin-28 (SS-28), respectively. One peak exhibited a higher molecular weight (about 10,000) and may contain a proform of somatostatin. Local injection of the neurotoxin kainic acid (1 microgram) into the left striatum resulted in a persistent decrease (65-85%) of all three forms of somatostatin. In the contralateral--not injected--striatum a decrease of SLI was also observed which was maximal (45%) after 2 days and was largely abolished after 7 days. This decrease of SLI in the contralateral striatum, however, was due mainly to a decrease of SS-14 and SS-28 but not of the putative proform. Our data suggest that kainic acid causes a destruction of somatostatin-containing perikarya in the injected striatum, whereas in the contralateral striatum increased release with subsequent inactivation of SS-14 and SS-28 takes place. The putative somatostatin proform may serve as neurochemical marker for somatostatin-containing perikarya in the striatum. |
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Authors:
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G Sperk; R Widmann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neurochemistry Volume: 45 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1985 Nov |
Date Detail:
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Created Date: 1985-11-18 Completed Date: 1985-11-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1441-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, High Pressure Liquid Corpus Striatum / drug effects, metabolism* Dose-Response Relationship, Drug Kainic Acid / pharmacology* Kinetics Male Protein Precursors / isolation & purification, metabolism* Radioimmunoassay Rats Rats, Inbred Strains Somatostatin / isolation & purification, metabolism* Somatostatin-28 |
| Chemical | |
Reg. No./Substance:
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0/Protein Precursors; 487-79-6/Kainic Acid; 51110-01-1/Somatostatin; 75037-27-3/Somatostatin-28 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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