Document Detail

Somatostatin analog RC-160 inhibits growth of CFPAC-1 human pancreatic cancer cells in vitro and intracellular production of cyclic adenosine monophosphate.
MedLine Citation:
PMID:  7860145     Owner:  NLM     Status:  MEDLINE    
The effect of somatostatin analog RC-160 on the growth of CFPAC-1 human pancreatic cancer cells in vitro was investigated. RC-160 effectively inhibited the proliferation of CFPAC-1 cells in culture, inducing a time- and dose-dependent decrease in the number of treated cells. A significant suppression of cell growth was observed after 48 and 72 hr of the exposure to (1 microM) RC-160, the cell number being decreased by 38% and 46%, respectively. RC-160 was more potent than SS-14 or SMS201-995 in inhibiting the growth of CFPAC-1 cells, and after 48-hr treatment the cell number decreased by 49% for RC-160 compared with 12% for SS-14 and 27% for SMS201-995. Binding experiments demonstrated that specific receptors for somatostatin were present on CFPAC-1 cells. SS-14 showed a high binding affinity for [125I]-Tyr11-SS-14 receptors on CFPAC-1 cells. Scatchard analysis indicated the presence of 2 classes of somatostatin binding sites on the cells, one with high binding affinity and low capacity and the other with low binding affinity and high capacity. RC-160 could bind to somatostatin receptors on these cells with an affinity similar to SS-14 but significantly higher than that of SMS201-995. Radioimmunoassay of intracellular cAMP showed that RC-160 could powerfully inhibit forskolin-stimulated cAMP production in CFPAC-1 cells. Addition of forskolin to the cultures increased cAMP concentrations in the cellular lysate of treated cells. RC-160 attenuated or nullified in a dose-dependent manner the cAMP production stimulated by forskolin. Our observations indicate that somatostatin analog RC-160 inhibits the proliferation of CFPAC-1 human pancreatic cancer cells in vitro and that this effect may involve the intracellular cAMP pathway.
Y Qin; T Ertl; K Groot; J Horvath; R Z Cai; A V Schally
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  60     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-03-23     Completed Date:  1995-03-23     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  694-700     Citation Subset:  IM    
Endocrine, Polypeptide and Cancer Institute, Veterans Affairs Medical Center, New Orleans, LA 70146..
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MeSH Terms
Amino Acid Sequence
Antineoplastic Agents / pharmacology*
Binding, Competitive
Carcinoma / drug therapy*,  metabolism,  pathology
Cell Division / drug effects
Cyclic AMP / biosynthesis*
Depression, Chemical
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Forskolin / pharmacology
Molecular Sequence Data
Neoplasm Proteins / metabolism
Octreotide / pharmacology
Pancreatic Neoplasms / drug therapy*,  metabolism,  pathology
Receptors, Somatostatin / drug effects,  metabolism
Signal Transduction / drug effects
Somatostatin / analogs & derivatives*,  pharmacology
Tumor Cells, Cultured / drug effects
Reg. No./Substance:
0/Antineoplastic Agents; 0/Neoplasm Proteins; 0/Receptors, Somatostatin; 0/vapreotide; 51110-01-1/Somatostatin; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; 83150-76-9/Octreotide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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