Document Detail


Somatostatin receptor subtypes in human type 2 diabetic islets.
MedLine Citation:
PMID:  20182388     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Somatostatin inhibits hormone release through 5 G protein-coupled somatostatin receptors (sst1-sst5). However, the role of somatostatin in islet physiology is not fully known. The immunoreactivity to sst1 to sst5 in normal human endocrine pancreas has been described. The present study reports the expression of sst1 to sst5 in human pancreatic islets with type 2 diabetes mellitus.
METHODS: Pancreatic autopsy specimens from individuals with type 2 diabetes mellitus and matched controls were double immunostained to demonstrate sst1 to sst5 in the major islet cell types.
RESULTS: Most apparent differences in type 2 diabetic islets were the lack of sst1 and sst4 in glucagon cells and sst1-3 and 4 in somatostatin cells, whereas minor changes were demonstrated in insulin cells. The pancreatic polypeptide cells showed a reversed staining pattern in diabetic islets compared with the controls.
CONCLUSIONS: In type 2 diabetes mellitus, the sst pattern differed from that of the controls in somatostatin, pancreatic polypeptide, and glucagon cells, to a minor extent in insulin cells. It is unclear whether the changes in sst patterns are primarily due to the diabetes or secondary to metabolic disturbances. However, this study may be the basis for further functional studies to evaluate the role of sst1 to sst5 in the diabetic state.
Authors:
Guida M Portela-Gomes; Lars Grimelius; Per Westermark; Mats Stridsberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pancreas     Volume:  39     ISSN:  1536-4828     ISO Abbreviation:  Pancreas     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-28     Completed Date:  2011-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8608542     Medline TA:  Pancreas     Country:  United States    
Other Details:
Languages:  eng     Pagination:  836-42     Citation Subset:  IM    
Affiliation:
Department of Gastroenterology, Lisbon University, Lisbon, Portugal. portela_gomes@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Autopsy
Diabetes Mellitus, Type 2 / metabolism*,  physiopathology
Female
Humans
Immunohistochemistry
Islets of Langerhans / metabolism*
Male
Pancreatic Polypeptide-Secreting Cells / metabolism
Protein Isoforms / metabolism
Receptors, Somatostatin / metabolism*
Somatostatin-Secreting Cells / metabolism
Chemical
Reg. No./Substance:
0/Protein Isoforms; 0/Receptors, Somatostatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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