Document Detail


Somatic and reproductive outcomes in mice treated with cyclophosphamide in pre-pubertal age.
MedLine Citation:
PMID:  23278118     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Disruption in the normal timing of female puberty, such as in pre-pubertal cancer treatments, can cause abnormal somatic development. We sought to evaluate the impact of cyclophosphamide (CTX) on the somatic, uterine, and ovarian, development of pre-pubertal mice. Pre-pubertal (day 18 of life) C57BL/6J female mice were randomized to receive placebo (group 1A and 1B), 200 mg/kg CTX (group 2A), or 120 mg/kg CTX (group 2B). Mice were euthanized on day 56 (A groups) or 95 (B groups) of life. Body weight and length, uterine and ovarian weight and right femur length and weight were measured, and ovarian insufficiency was assessed. Data were analyzed using ANOVA and t-test. Body weight and length did not differ among groups at time of euthanasia. The femur was shorter and weighed less in mice treated with CTX than in controls. Uterine weight was lower in group 2B than 1B (46.1 mg, 95% CI: 42.9-49.4, vs. 62.2 mg, 95% CI: 58.5-65.8, respectively; p = 0.005) and was lower in mice that developed ovarian insufficiency than in mice that did not (p < 0.05). Ovarian weight was lower in mice treated with CTX, regardless of whether they developed ovarian insufficiency. Even with no observable effect on adult body length and weight, CTX treatment in pre-pubertal mice appears to negatively affect femur, uterine, and ovarian development. However, uterine development seems to be dependent on the hormonal status created by CTX more than on its direct effect.
Authors:
Laura Detti; Daniel C Martin; Robert W Williams; Natalia Schlabritz-Loutsevich; Lucy J Williams; Rebecca A Uhlmann
Related Documents :
21415388 - Sdf-1α induction in mature smooth muscle cells by inactivation of pten is a critical m...
10037158 - Human nerve growth factor beta (hngf-beta): mammary gland specific expression and produ...
10882298 - Transgenic mice reveal roles for tgfalpha and egf receptor in mammary gland development...
18997818 - Pten deficiency accelerates tumour progression in a mouse model of thyroid cancer.
3119398 - Human embryonic hemopoiesis: control mechanisms underlying progenitor differentiation i...
19709448 - Lupin protein isolate and cysteine-supplemented casein reduce calcification of atherosc...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-2
Journal Detail:
Title:  Systems biology in reproductive medicine     Volume:  -     ISSN:  1939-6376     ISO Abbreviation:  Syst Biol Reprod Med     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101464963     Medline TA:  Syst Biol Reprod Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
University of Tennessee Health Science Center , Department of Obstetrics and Gynecology.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  An Overview of the Modulatory Effects of Oleic Acid in Health and Disease.
Next Document:  Dopamine D1 Receptor Signaling System Regulates Ryanodine Receptor Expression in Ethanol Physical De...