| Somatic nucleus reprogramming is significantly improved by m-carboxycinnamic acid bishydroxamide, a histone deacetylase inhibitor. | |
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MedLine Citation:
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PMID: 20566633 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Somatic cell nuclear transfer (SCNT) has shown tremendous potential for understanding the mechanisms of reprogramming and creating applications in the realms of agriculture, therapeutics, and regenerative medicine, although the efficiency of reprogramming is still low. Somatic nucleus reprogramming is triggered in the short time after transfer into recipient cytoplasm, and therefore, this period is regarded as a key stage for optimizing SCNT. Here we report that CBHA, a histone deacetylase inhibitor, modifies the acetylation status of somatic nuclei and increases the developmental potential of mouse cloned embryos to reach pre- and post-implantation stages. Furthermore, the cloned embryos treated by CBHA displayed higher efficiency in the derivation of nuclear transfer embryonic stem cell lines by promoting outgrowths. More importantly, CBHA increased blastocyst quality compared with trichostatin A, another prevalent histone deacetylase inhibitor reported previously. Use of CBHA should improve the productivity of SCNT for a variety of research and clinical applications, and comparisons of cells with different levels of pluripotency and treated with CBHA versus trichostatin A will facilitate studies of the mechanisms of reprogramming. |
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Authors:
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Xiangpeng Dai; Jie Hao; Xiao-Jun Hou; Tang Hai; Yong Fan; Yang Yu; Alice Jouneau; Liu Wang; Qi Zhou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-21 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 285 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-27 Completed Date: 2010-10-26 Revised Date: 2012-05-07 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 31002-10 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylation Animals Blastocyst / cytology, metabolism* Cell Dedifferentiation / drug effects* Cell Nucleus / metabolism* Cinnamates / pharmacology* Cloning, Organism* Female Histone Deacetylase Inhibitors / pharmacology* Hydroxamic Acids / pharmacology Male Mice Mice, Inbred ICR Nuclear Transfer Techniques |
| Chemical | |
Reg. No./Substance:
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0/Cinnamates; 0/Histone Deacetylase Inhibitors; 0/Hydroxamic Acids; 0/carboxycinnamic acid bishydroxamide; 58880-19-6/trichostatin A |
| Comments/Corrections | |
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