Document Detail


Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants.
MedLine Citation:
PMID:  16049496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Caenorhabditis elegans homologues of the retinoblastoma (Rb) tumour suppressor complex specify cell lineage during development. Here we show that mutations in Rb pathway components enhance RNA interference (RNAi) and cause somatic cells to express genes and elaborate perinuclear structures normally limited to germline-specific P granules. Furthermore, particular gene inactivations that disrupt RNAi reverse the cell lineage transformations of Rb pathway mutants. These findings suggest that mutations in Rb pathway components cause cells to revert to patterns of gene expression normally restricted to germ cells. Rb may act by a similar mechanism to transform mammalian cells.
Authors:
Duo Wang; Scott Kennedy; Darryl Conte; John K Kim; Harrison W Gabel; Ravi S Kamath; Craig C Mello; Gary Ruvkun
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nature     Volume:  436     ISSN:  1476-4687     ISO Abbreviation:  Nature     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-28     Completed Date:  2005-08-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0410462     Medline TA:  Nature     Country:  England    
Other Details:
Languages:  eng     Pagination:  593-7     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Animals
Caenorhabditis elegans / cytology*,  genetics
Caenorhabditis elegans Proteins / genetics,  metabolism
Cell Differentiation
Cell Lineage
Cytoplasmic Granules / metabolism*
Exoribonucleases / genetics,  metabolism
Female
Gene Expression Regulation, Developmental
Genes, Helminth / genetics
Germ Cells / cytology*,  metabolism*
Mutation / genetics*
Phenotype
RNA Interference*
RNA Replicase / genetics,  metabolism
Retinoblastoma / metabolism*
Transcription Factors / genetics,  metabolism
Transgenes / genetics
Vulva / cytology,  metabolism,  pathology
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/Transcription Factors; 0/lin-15B protein, C elegans; EC 2.7.7.48/RNA Replicase; EC 2.7.7.48/RNA-directed RNA polymerase RRF-3, C elegans; EC 3.1.-/ERI-1 protein, C elegans; EC 3.1.-/Exoribonucleases
Comments/Corrections
Comment In:
Nature. 2005 Jul 28;436(7050):473-4   [PMID:  16049464 ]

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