| Somatic hybridization between human and mouse lymphoblast cells produced by an electric pulse-induced fusion technique. | |
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MedLine Citation:
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PMID: 6498948 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The technique of electric pulse-induced cell fusion (electro-fusion) was used to obtain heterokaryons between normal human lymphoblasts (HSC93) and mouse leukemic lymphoblasts (MCN151). The two types of cells were brought into contact in the cell suspension by dielectrophoresis with an alternating electric field (0.8 kV/cm, 100 kHz) in the presence of calcium ions and pronase E. Cell fusion was induced by giving two successive electric pulses (3.3 and 5 kV/cm, 10 microsec). Prior treatment of human (but not mouse) lymphoblasts with neuraminidase improved fusion efficiency. Differential staining of the two types of cells with Janus Green and Neutral Red showed that about 40% of the viable fused cells underwent heterokaryonic fusion. We concluded that electrofusion is an efficient method for obtaining heterokaryons from human and mouse lymphoblasts. |
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Authors:
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T Ohno-Shosaku; H Hama-Inaba; Y Okada |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell structure and function Volume: 9 ISSN: 0386-7196 ISO Abbreviation: Cell Struct. Funct. Publication Date: 1984 Jun |
Date Detail:
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Created Date: 1985-01-03 Completed Date: 1985-01-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7608465 Medline TA: Cell Struct Funct Country: JAPAN |
Other Details:
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Languages: eng Pagination: 193-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Fusion Electric Stimulation Humans Hybrid Cells / physiology* Leukemia L5178 / physiopathology* Leukemia, Experimental / physiopathology* Lymphocytes / physiology* Mice Mutation |
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