Document Detail


Somatic antigens of Shigella. The strucuture of the specific polysaccharide chain of Shigella dysenteriae type 5 lipopolysaccharide.
MedLine Citation:
PMID:  334537     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The specific polysaccharide was released from Shigella dysenteriae type 5 lipopolysaccharide by mild acidic hydrolysis and then purified by gel chromatography on Sephadex G-50. The polysaccharide was built up of residues of D-mannose, 2-acetamido-2-deoxy-D-glucose, 3-0-(D-1-carboxyethyl)-L-rhamnose (rhamnolactylic acid) and 0-acetyl groups in a ratio 2:1:1:1. On the basis of radiospectroscopy, methylation analysis, Smith degradation, and chromium trioxide oxidation, the repeating oligosaccharide unit of the polysaccharide can be assigned the following structure: (formula: see text) where GlcNAc is 2-acetamido-2-deoxy-D-glucopyranose, Manp is mannopyranose, RhaLcA is rhammolacytic acid and Ac is an acetyl group. The serological properties of Sh. dysenteriae somatic antigens are discussed in relation to the chemical structures of their specific polysaccharides.
Authors:
B A Dmitriev; L V Backinowsky; Y A Knirel; N K Kochetkov
Related Documents :
2776127 - Structure of the capsular polysaccharide of klebsiella ozaenae serotype k4 containing 3...
14006997 - Isolation and partial characterization of a capsular material from staphylococcus aureus.
11376607 - Structure of the o-specific polysaccharide of vibrio cholerae o9 containing 2-acetamido...
1499017 - Evidence that the acidic polysaccharide secreted by agrobacterium radiobacter (atcc 532...
569427 - Nutritional improvement of food proteins by means of the plastein reaction and its nove...
3040657 - Thoughts on fiber utilization in swine.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  78     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1977 Sep 
Date Detail:
Created Date:  1977-12-29     Completed Date:  1977-12-29     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  381-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Carbohydrates / analysis
Lipopolysaccharides* / immunology
Magnetic Resonance Spectroscopy
Mass Spectrometry
Molecular Conformation
Polysaccharides, Bacterial* / isolation & purification
Shigella dysenteriae / immunology*
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Lipopolysaccharides; 0/Polysaccharides, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Affinity chromatography on agarose-hexyl-adenosine-5'-phosphate of methionyl-tRNA synthetase from Es...
Next Document:  Ternary complex formation between elongation factor Tu, GTP and aminoacyl-tRNA: an equilibrium study...