Document Detail

Somatic antigens of Shigella. The strucuture of the specific polysaccharide chain of Shigella dysenteriae type 5 lipopolysaccharide.
MedLine Citation:
PMID:  334537     Owner:  NLM     Status:  MEDLINE    
The specific polysaccharide was released from Shigella dysenteriae type 5 lipopolysaccharide by mild acidic hydrolysis and then purified by gel chromatography on Sephadex G-50. The polysaccharide was built up of residues of D-mannose, 2-acetamido-2-deoxy-D-glucose, 3-0-(D-1-carboxyethyl)-L-rhamnose (rhamnolactylic acid) and 0-acetyl groups in a ratio 2:1:1:1. On the basis of radiospectroscopy, methylation analysis, Smith degradation, and chromium trioxide oxidation, the repeating oligosaccharide unit of the polysaccharide can be assigned the following structure: (formula: see text) where GlcNAc is 2-acetamido-2-deoxy-D-glucopyranose, Manp is mannopyranose, RhaLcA is rhammolacytic acid and Ac is an acetyl group. The serological properties of Sh. dysenteriae somatic antigens are discussed in relation to the chemical structures of their specific polysaccharides.
B A Dmitriev; L V Backinowsky; Y A Knirel; N K Kochetkov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  78     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1977 Sep 
Date Detail:
Created Date:  1977-12-29     Completed Date:  1977-12-29     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  381-7     Citation Subset:  IM    
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MeSH Terms
Carbohydrates / analysis
Lipopolysaccharides* / immunology
Magnetic Resonance Spectroscopy
Mass Spectrometry
Molecular Conformation
Polysaccharides, Bacterial* / isolation & purification
Shigella dysenteriae / immunology*
Reg. No./Substance:
0/Carbohydrates; 0/Lipopolysaccharides; 0/Polysaccharides, Bacterial

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