| Solvent dependence of the rotational diffusion of TOAC-spin-labeled alamethicin. | |
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MedLine Citation:
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PMID: 17589865 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three derivatives of the hydrophobic, channel-forming peptaibiotic alamethicin (F50/5) have been synthesized, the original Aib residue at position 1, 8, or 16 being replaced with the spin-labeled amino acid TOAC (=2,2,6,6-tetramethylpiperidin-1-oxyl-4-amino-4-carboxylic acid). Electron-paramagnetic-resonance (EPR) spectroscopy was used to characterize the rotational diffusion of these compounds in five isotropic solvents of differing viscosity and polarity, including MeOH, EtOH, PrOH, i-PrOH, and hexanol (HxOH). In MeOH, the labeled alamethicins were found to rotate anisotropically as a monomer (axial ratio a/b=3). In aliphatic alcohols of increasing viscosity (and hydrophobicity), the rotational correlation times progressively increased. Even in HxOH, the (fivefold) increase in correlation time was no greater than the increase in viscosity. We conclude that TOAC-labeled alamethicins remain monomeric in these solvents of relatively high polarity. |
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Authors:
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Derek Marsh; Micha Jost; Cristina Peggion; Claudio Toniolo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chemistry & biodiversity Volume: 4 ISSN: 1612-1880 ISO Abbreviation: Chem. Biodivers. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-06-28 Completed Date: 2007-07-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101197449 Medline TA: Chem Biodivers Country: Switzerland |
Other Details:
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Languages: eng Pagination: 1269-74 Citation Subset: IM |
Affiliation:
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Max-Planck-Institut für biophysikalische Chemie, Abteilung Spektroskopie, Göttingen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alamethicin
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chemistry* Cyclic N-Oxides / chemistry* Electron Spin Resonance Spectroscopy Solvents / chemistry* Spin Labels* |
| Chemical | |
Reg. No./Substance:
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0/Cyclic N-Oxides; 0/Solvents; 0/Spin Labels; 15871-57-5/2,2,6,6-tetramethylpiperidine-N-oxide-4-amino-4-carboxylic acid; 27061-78-5/Alamethicin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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