Document Detail


Solution synthesis of human neuropeptide Y (hNPY).
MedLine Citation:
PMID:  2805172     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human neuropeptide Y (hNPY) was synthesized in a conventional manner by assembling seven peptide fragments followed by reduction of the Met(O) residue with phenylthiotrimethylsilane and subsequent deprotection with 1 M trimethylsilyl trifluoromethanesulfonate (TMSOTf)-thioanisole in trifluoroacetic acid (TFA). Alternatively, deprotection was performed in a two-step manner; first, treatment with 1 M trimethylsilyl bromide-thioanisole in TFA, and then with 1 M TMSOTf-thioanisole in TFA. After purification by gel-filtration on Sephadex G-25, followed by reversed-phase high-performance liquid chromatography, a highly purified sample of synthetic hNPY was obtained in both cases. When administered in dogs, synthetic hNPY was as active as porcine NPY in terms of the effects on systemic arterial blood pressure, pancreatic blood flow, and superior mesentric artery (SMA) blood flow. Met(O)17-hNPY was found to be as active as the parent sample in these bioassays.
Authors:
S Ono; K Kitagawa; S Futaki; S Kiyama; Y Ooi; T Akita; S Higashide; K Inoue; S Sumi; T Tobe
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemical & pharmaceutical bulletin     Volume:  37     ISSN:  0009-2363     ISO Abbreviation:  Chem. Pharm. Bull.     Publication Date:  1989 Jul 
Date Detail:
Created Date:  1989-12-12     Completed Date:  1989-12-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0377775     Medline TA:  Chem Pharm Bull (Tokyo)     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1925-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Humans
Molecular Sequence Data
Neuropeptide Y / chemical synthesis*
Chemical
Reg. No./Substance:
0/Neuropeptide Y

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