| Solution synthesis of human neuropeptide Y (hNPY). | |
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MedLine Citation:
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PMID: 2805172 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human neuropeptide Y (hNPY) was synthesized in a conventional manner by assembling seven peptide fragments followed by reduction of the Met(O) residue with phenylthiotrimethylsilane and subsequent deprotection with 1 M trimethylsilyl trifluoromethanesulfonate (TMSOTf)-thioanisole in trifluoroacetic acid (TFA). Alternatively, deprotection was performed in a two-step manner; first, treatment with 1 M trimethylsilyl bromide-thioanisole in TFA, and then with 1 M TMSOTf-thioanisole in TFA. After purification by gel-filtration on Sephadex G-25, followed by reversed-phase high-performance liquid chromatography, a highly purified sample of synthetic hNPY was obtained in both cases. When administered in dogs, synthetic hNPY was as active as porcine NPY in terms of the effects on systemic arterial blood pressure, pancreatic blood flow, and superior mesentric artery (SMA) blood flow. Met(O)17-hNPY was found to be as active as the parent sample in these bioassays. |
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Authors:
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S Ono; K Kitagawa; S Futaki; S Kiyama; Y Ooi; T Akita; S Higashide; K Inoue; S Sumi; T Tobe |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chemical & pharmaceutical bulletin Volume: 37 ISSN: 0009-2363 ISO Abbreviation: Chem. Pharm. Bull. Publication Date: 1989 Jul |
Date Detail:
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Created Date: 1989-12-12 Completed Date: 1989-12-12 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0377775 Medline TA: Chem Pharm Bull (Tokyo) Country: JAPAN |
Other Details:
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Languages: eng Pagination: 1925-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Humans Molecular Sequence Data Neuropeptide Y / chemical synthesis* |
| Chemical | |
Reg. No./Substance:
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0/Neuropeptide Y |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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