Document Detail

Solution structure and RNA interactions of the RNA recognition motif from eukaryotic translation initiation factor 4B.
MedLine Citation:
PMID:  12885229     Owner:  NLM     Status:  MEDLINE    
Eukaryotic initiation factor 4B (eIF4B) is a multidomain protein with a range of activities that serves primarily to promote association of messenger RNA to the 40S ribosomal subunit during translation initiation. We report here the solution structure of the eIF4B RNA recognition motif (RRM) domain. It adopts a classical RRM fold, with a beta alpha beta beta alpha beta topology. The most striking difference with other RRM structures is in the disposition of loop 3, which connects the beta 2 and beta 3 strands and is implicated in RNA recognition. This loop folds down against the body of the RRM and exhibits restricted motion on a milli- to microsecond time scale. Although it contributes to a large basic patch on the RNA binding surface, it does not protrude out from the domain as observed in other RRM structures, possibly implying a different mode of RNA binding. On its own, the core RRM domain provides only a relative weak interaction with RNA targets and appears to require extensions at the N- and C-terminus for high-affinity binding.
Keiran Fleming; Jamie Ghuman; Xuemei Yuan; Peter Simpson; Andrea Szendröi; Stephen Matthews; Stephen Curry
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry     Volume:  42     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-07-29     Completed Date:  2003-11-06     Revised Date:  2009-01-12    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8966-75     Citation Subset:  IM    
Department of Biological Sciences and Centre for Structural Biology, Imperial College London, Wolfson Laboratory, Exhibition Road, London SW7 2AY, UK.
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MeSH Terms
Amino Acid Motifs
Amino Acid Sequence
Eukaryotic Initiation Factors / chemistry*,  metabolism
Models, Molecular
Molecular Sequence Data
Nuclear Magnetic Resonance, Biomolecular
Protein Conformation
Protein Structure, Secondary
Protein Structure, Tertiary
RNA / chemistry*,  metabolism
RNA-Binding Proteins / chemistry*,  metabolism
Sequence Homology, Amino Acid
Grant Support
JE514316//Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Eukaryotic Initiation Factors; 0/RNA-Binding Proteins; 0/Solutions; 0/eIF-4B; 63231-63-0/RNA

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