| Solution structure of the HRDC domain of human Bloom syndrome protein BLM. | |
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MedLine Citation:
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PMID: 20739603 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Bloom syndrome is a rare genetic disorder characterized by severe growth retardation and cancer predisposition. The disease is caused by a loss of function of the Bloom syndrome protein (BLM), a member of the RecQ family of DNA helicases. Here we report on the first 3D structure of a BLM fragment, a solution structure of the C-terminal helicase-and-ribonuclease D-C-terminal (HRDC) domain from human BLM. The structure reveals unique features of BLM HRDC that are distinct from the HRDC domain of Werner syndrome protein. In particular, BLM HRDC retains many acidic residues exposed to the solvent, which makes the domain surface extensively electronegative. Consistent with this, fluorescence polarization assays showed an inability of isolated BLM HRDC to interact with DNA substrates. Analyses employing ultracentrifugation, gel-filtration, CD spectroscopy and dynamic light scattering showed that the BLM HRDC domain exists as a stable monomer in solution. The results show that BLM HRDC is a compact, robust and acidic motif which may play a distinct role apart from DNA binding. |
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Authors:
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Akiko Sato; Masaki Mishima; Aki Nagai; Sun-Yong Kim; Yutaka Ito; Toshio Hakoshima; Jun-Goo Jee; Ken Kitano |
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Publication Detail:
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Type: Journal Article Date: 2010-08-25 |
Journal Detail:
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Title: Journal of biochemistry Volume: 148 ISSN: 1756-2651 ISO Abbreviation: J. Biochem. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-06 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: England |
Other Details:
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Languages: eng Pagination: 517-25 Citation Subset: IM |
Affiliation:
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Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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PDB/2RRD |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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