Document Detail

Solute diffusion through stripped mouse duodenum.
MedLine Citation:
PMID:  18195487     Owner:  NLM     Status:  MEDLINE    
We measured villous cell intracellular pH (pH(i)) and solute diffusion between the bathing media and the epithelial cells in stripped, chambered mouse duodenum. Apical perfusion of a high CO2 solution rapidly acidified the upper villous cells with recovery after its removal. Apical zoniporide (ZP) enhanced CO(2)-induced acidification. Serosal ZP, dimethylamiloride (DMA) or stilbene anion transport inhibitors failed to alter CO(2)-induced acidification, whereas serosal high CO(2) buffer acidified the upper villous cells. Serosal 5-hydroxytryptamine rapidly acidified the upper villous cells. All serosally-perfused fluorescent compounds stained the crypt area, but not the villi or villous cells. In contrast, intravenous carboxyfluorescein quickly diffused into the interstitial space of the entire mucosa, and mucosally perfused fluorescent compound rapidly penetrated the epithelial cell layer. In muscle-stripped duodenum mounted in a small-aperture perfusion chamber, serosal solutes can readily diffuse only to the crypt cell region, whereas access to the villous epithelial cells is diffusion-limited. In contrast, rapid villous cell responses to serosally applied solutes are best explained by neural reflexes. Limited viability of the villous cells and impaired structural stability of the villi further limit long-term, villous cell functional studies of mucosal preparations mounted in small aperture diffusion chambers.
T Takeuchi; M Ham; M Mizumori; P H Guth; E Engel; J D Kaunitz; Y Akiba
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of physiology and pharmacology : an official journal of the Polish Physiological Society     Volume:  58     ISSN:  1899-1505     ISO Abbreviation:  J. Physiol. Pharmacol.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2008-01-15     Completed Date:  2008-04-24     Revised Date:  2008-05-19    
Medline Journal Info:
Nlm Unique ID:  9114501     Medline TA:  J Physiol Pharmacol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  767-91     Citation Subset:  IM    
Greater Los Angles Veterans Affairs Healthcare System, University of California Los Angeles, Los Angeles, CA 90073, USA.
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MeSH Terms
Amiloride / analogs & derivatives,  pharmacology
Biological Transport / drug effects
Carbon Dioxide / metabolism
Duodenum / metabolism*
Epithelial Cells / drug effects,  metabolism
Fluoresceins / metabolism
Fluorescent Dyes / metabolism
Guanidines / pharmacology
Hydrogen-Ion Concentration
Intestinal Mucosa / metabolism*
Mice, Inbred C57BL
Pyrazoles / pharmacology
Serotonin / pharmacology
Sodium-Bicarbonate Symporters / antagonists & inhibitors
Sodium-Hydrogen Antiporter / antagonists & inhibitors
Stilbenes / metabolism
Reg. No./Substance:
0/(1-(quinolin-5-yl)-5-cyclopropyl-1H-pyrazole-4-carbonyl)guanidine; 0/Anions; 0/Fluoresceins; 0/Fluorescent Dyes; 0/Guanidines; 0/Pyrazoles; 0/Slc4a4 protein, mouse; 0/Sodium-Bicarbonate Symporters; 0/Sodium-Hydrogen Antiporter; 0/Solutions; 0/Stilbenes; 0/growth factor-activatable Na-H exchanger NHE-1; 1214-79-5/5-dimethylamiloride; 124-38-9/Carbon Dioxide; 2609-46-3/Amiloride; 3301-79-9/6-carboxyfluorescein; 50-67-9/Serotonin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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