| Solute absorption from the airways of the isolated rat lung. IV. Mechanisms of absorption of fluorophore-labeled poly-alpha,beta-[N(2-hydroxyethyl)-DL-aspartamide] | |
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MedLine Citation:
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PMID: 8165179 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The pulmonary absorption kinetics of a single molecular weight distribution (MWD) of fluorophore-labeled poly-alpha,beta-[N(2-hydroxyethyl)-DL-aspartamide] (F-PHEA), a hydrophilic and biocompatible synthetic polypeptide, were studied in the isolated, perfused rat lung (iprl) as functions of administered polymer concentration, dose, vehicle, and presence and absence of fluorophore. The MWD was characterized before and after absorption by measurement of weight- and number-averaged molecular weights (Mw and Mn, respectively) using high-performance gel-permeation chromatography. Values for Mw and Mn were 8.6 and 5.3 kD before, and 6.7 and 4.7 kD after, absorption into the perfusate; there was no significant metabolism and the MWD of the absorbed polymer was independent of both dose and sampling time over a 3-hr period. F-PHEA failed to show any evidence of aggregation in solution or changes in dose distribution within the airways as functions of increasing polymer concentration and dose. A concentration ranging study indicated the presence of a saturable, carrier-mediated transport process for F-PHEA with a maximum absorption rate, Vmax, of approximately 180 micrograms or 0.027 mumol/hr. Coadministration of fluorophore-free PHEA was capable of depressing the absorption of F-PHEA. The transport process for F-PHEA appeared to have a molecular weight limit of about 7 kD for this hydrophilic polymer. |
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Authors:
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P R Byron; Z Sun; H Katayama; F Rypacek |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pharmaceutical research Volume: 11 ISSN: 0724-8741 ISO Abbreviation: Pharm. Res. Publication Date: 1994 Feb |
Date Detail:
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Created Date: 1994-05-24 Completed Date: 1994-05-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8406521 Medline TA: Pharm Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 221-5 Citation Subset: IM |
Affiliation:
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Aerosol Research Group, School of Pharmacy, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0533. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Animals Dose-Response Relationship, Drug Drug Carriers Fluorescent Dyes Lung / metabolism* Molecular Weight Peptides / administration & dosage, pharmacokinetics* Polymers Rats Regression Analysis Tissue Distribution Trachea / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Drug Carriers; 0/Fluorescent Dyes; 0/Peptides; 0/Polymers; 0/alpha,beta-poly((2-hydroxyethyl)-aspartamide) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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