Document Detail


Soluble, thrombin-related material in arterial thrombi and plasma studied during catheter-directed intra-arterial thrombolysis.
MedLine Citation:
PMID:  10192657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated soluble, thrombin-related material in arterial thrombi and venous plasma during catheter-directed thrombolysis with alteplase. Arteriography was performed before thrombolysis and 0.5, 3, 10 and 24 h after the onset of treatment in six patients with (sub)acute lower extremity ischaemia caused by native artery or bypass occlusion. Samples were collected simultaneously from the thrombus and venous blood. After adding inhibitors of thrombin and plasmin, the centrifuged samples were assayed for prothrombin fragment 1+2 (F1+2), thrombin-antithrombin complex (TAT) and fibrinopeptide A (FPA). Levels of F1+2, TAT and FPA were extremely elevated in the thrombus-related samples before the blood flow was re-established (at 0 and 0.5 h) in all five successfully treated patients. In comparison, venous plasma levels of F1+2, TAT and FPA were moderately elevated, and reached a maximum at 3 h. In conclusion, material aspirated from lysing human thrombi formed in vivo contains large amounts of F1+2, TAT and FPA, but our methods prevented us from detecting enzymatically active thrombin.
Authors:
G Sandbaek; S Bjørnsen; F Brosstad
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis     Volume:  10     ISSN:  0957-5235     ISO Abbreviation:  Blood Coagul. Fibrinolysis     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-06-17     Completed Date:  1999-06-17     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9102551     Medline TA:  Blood Coagul Fibrinolysis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  87-91     Citation Subset:  IM    
Affiliation:
Research Institute for Internal Medicine, Rikshospitalet, The National Hospital, University of Oslo, Norway. gunnar.sandbaek@rh.uio.no
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MeSH Terms
Descriptor/Qualifier:
Aged
Angiography
Antithrombin III / analysis*
Blood Coagulation Factors / analysis*
Catheterization
Female
Fibrinolytic Agents / therapeutic use
Fibrinopeptide A / analysis
Humans
Ischemia / drug therapy
Leg / blood supply
Male
Middle Aged
Peptide Fragments / analysis
Peptide Hydrolases / analysis*
Protein Precursors / analysis
Prothrombin / analysis
Solubility
Thrombolytic Therapy*
Thrombosis / blood*,  drug therapy*
Time Factors
Tissue Plasminogen Activator / therapeutic use
Treatment Outcome
Chemical
Reg. No./Substance:
0/Blood Coagulation Factors; 0/Fibrinolytic Agents; 0/Peptide Fragments; 0/Protein Precursors; 0/antithrombin III-protease complex; 25422-31-5/Fibrinopeptide A; 72270-84-9/prothrombin fragment 1; 78768-79-3/prothrombin fragment 2; 9000-94-6/Antithrombin III; 9001-26-7/Prothrombin; EC 3.4.-/Peptide Hydrolases; EC 3.4.21.68/Tissue Plasminogen Activator

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