Document Detail


Soluble and immobilized catalase. Effect of pressure and inhibition on kinetics and deactivation.
MedLine Citation:
PMID:  7847895     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This article examines the effect of pressure on the steady-state kinetics and long-term deactivation of the enzyme catalase supported on porous alumina. The reaction studied is the decomposition of hydrogen peroxide. The results of studies carried out in a continuous stirred-tank reactor under isothermal conditions are presented and compared with results obtained for soluble catalase. For soluble catalase, it is found that in the range of pressures studied, the oxygen flow rate increases with increase in pressure up to a certain value and then decreases. Hydrogen peroxide concentration appears to have a strong influence on pressure effects. With immobilized catalase, the pressure effects are not as prominent. Fluorescent microscopy studies of the immobilized enzyme suggest that this is probably because of pore diffusional limitations.
Authors:
P T Vasudevan; D S Thakur
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  49     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-03-07     Completed Date:  1995-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  173-89     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering, University of New Hampshire, Durham 03824.
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MeSH Terms
Descriptor/Qualifier:
Aluminum Oxide
Animals
Catalase / antagonists & inhibitors*,  metabolism*
Cattle
Enzyme Activation
Enzymes, Immobilized*
Fluorescein-5-isothiocyanate
Fluorescent Dyes
Hydrogen-Ion Concentration
Kinetics
Liver / enzymology
Oxygen / metabolism
Pressure
Solubility
Temperature
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Fluorescent Dyes; 1344-28-1/Aluminum Oxide; 3326-32-7/Fluorescein-5-isothiocyanate; 7782-44-7/Oxygen; EC 1.11.1.6/Catalase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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