Document Detail


Soluble fibrin species in arterial thrombi.
MedLine Citation:
PMID:  10691094     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to characterize soluble fibrin(ogen) species in human, arterial, in-vivo-formed thrombi, using the immunoblotting technique. Specimens were collected via intra-arterial catheters in six patients scheduled for catheter-directed thrombolysis. Unreduced and reduced samples of the supernatants from the arterial thrombi-derived specimens were electrophoresed on polyacrylamide gels and immunoblotted, using specific mono- and polyclonal anti-fibrin(ogen) antibodies. The reduced samples disclosed substantial amounts of high molecular weight material, consistent with alpha-chain polymers and gammagamma-dimers, as well as lower molecular weight material, such as alpha-, beta- and gamma-chains. No fibrinogen with intact fibrinopeptide A was detectable, and des-AABB fibrin represented a major fibrin derivative in the soluble part of the arterial thrombi. The alpha-chains were C-terminally degraded, most of them distal to position 259. In conclusion, we have demonstrated the presence of cross-linked fibrin derivatives in soluble, arterial thrombus-related material, without signs of fibrinogen-fibrin hybrids. The fibrin derivatives were C-terminally degraded, thus representing X-oligomeric material, most probably originating from plasmin degradation of insoluble thrombus fibrin. The present study supports the hypothesis of a dynamic equilibrium between clotting and lysis in thrombi.
Authors:
G Sandbaek; S Bjørnsen; J H Sobel; W Nieuwenhuizen; G Matsueda; F Brosstad
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis     Volume:  11     ISSN:  0957-5235     ISO Abbreviation:  Blood Coagul. Fibrinolysis     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-03-23     Completed Date:  2000-03-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9102551     Medline TA:  Blood Coagul Fibrinolysis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1-5     Citation Subset:  IM    
Affiliation:
Research Institute for Internal Medicine, The National Hospital (Rikshospitalet), University of Oslo, Norway. gunnar.sandbaek@rh.uio.no
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MeSH Terms
Descriptor/Qualifier:
Aged
Antibodies, Monoclonal / diagnostic use
Arteries
Blotting, Western
Female
Fibrin / chemistry*
Fibrinogen / analysis,  chemistry,  immunology
Fibrinopeptide A / analysis,  chemistry,  immunology
Humans
Male
Middle Aged
Molecular Weight
Oxidation-Reduction
Protein Structure, Quaternary
Solubility
Thrombosis / blood,  metabolism*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 25422-31-5/Fibrinopeptide A; 9001-31-4/Fibrin; 9001-32-5/Fibrinogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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