Document Detail


Soluble factors from Plasmodium falciparum-infected erythrocytes induce apoptosis in human brain vascular endothelial and neuroglia cells.
MedLine Citation:
PMID:  18848585     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The severity of malaria is multi-factorial. It is associated with parasite-induced alteration in pro-inflammatory and anti-inflammatory cytokine and chemokine levels in host serum and cerebrospinal fluid. It is also associated with sequestration and cytoadherence of parasitized erythrocytes (pRBCs) in post-capillary venules and blood-brain barrier (BBB) dysfunction. The role of these factors in development of vascular injury and tissue damage in malaria patients is unclear. While some studies indicate a requirement for pRBC adhesion to vascular endothelial cells (ECs) in brain capillaries to induce apoptosis and BBB damage, others show no role of apoptosis resulting from adhesion of pRBC to EC. In the present study, the hypothesis that soluble factors from Plasmodium falciparum-infected erythrocytes induce apoptosis in human brain vascular endothelial (HBVEC) and neuroglia cells (cellular components of the BBB) was tested. Apoptotic effects of parasitized (pRBC) and non-parasitized erythrocyte (RBC) conditioned medium on HBVEC and neuroglia cells were determined in vitro by evaluating nuclear DNA fragmentation (TUNEL assay) in cultured cells. Soluble factors from P. falciparum-infected erythrocytes in conditioned medium induced extensive DNA fragmentation in both cell lines, albeit to a greater extent in HBVEC than neuroglia, indicating that extended exposure to high levels of these soluble factors in serum may be associated with vascular, neuronal and tissue injury in malaria patients.
Authors:
Nana O Wilson; Ming-Bo Huang; Winston Anderson; Vincent Bond; Michael Powell; Winston E Thompson; Henry B Armah; Andrew A Adjei; Richard Gyasi; Yao Tettey; Jonathan K Stiles
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-09-19
Journal Detail:
Title:  Molecular and biochemical parasitology     Volume:  162     ISSN:  0166-6851     ISO Abbreviation:  Mol. Biochem. Parasitol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-10-28     Completed Date:  2009-02-04     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  8006324     Medline TA:  Mol Biochem Parasitol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  172-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Blood-Brain Barrier / metabolism
Brain / blood supply,  cytology*,  metabolism
Cells, Cultured
Endothelial Cells / metabolism,  parasitology
Endothelium, Vascular / cytology*
Erythrocytes / parasitology*
Humans
Neuroglia / cytology*,  metabolism,  parasitology
Plasmodium falciparum / metabolism*,  pathogenicity*
Grant Support
ID/Acronym/Agency:
G12 RR003034/RR/NCRR NIH HHS; G12 RR003034-226592/RR/NCRR NIH HHS; G12 RR003034-235449/RR/NCRR NIH HHS; R21 TW006804/TW/FIC NIH HHS; R21 TW006804-02/TW/FIC NIH HHS; R21 TW006804-02S1/TW/FIC NIH HHS; R21TW006804-01/TW/FIC NIH HHS; R25 GM058268/GM/NIGMS NIH HHS; R25 GM058268-11/GM/NIGMS NIH HHS; RR03034/RR/NCRR NIH HHS; S06GM08248/GM/NIGMS NIH HHS
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