Document Detail


Soluble factor-mediated differentiation of CD4+CD8+ thymocytes to single positives in vitro.
MedLine Citation:
PMID:  12366378     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although the thymic microenvironment provides the necessary elements for T-cell differentiation, the precise role of individual components remains to be determined. In this paper, attempts were made to address the possibility that CD4 or CD8 single-positive (SP) thymocytes could be developed from immature CD4+CD8+ (double-positive; DP) thymocytes in a suspension culture in the presence of soluble factors. We observed that IL-4 and IFN-gamma weakly induced DP cells to differentiate to CD4 cells, but not to CD8. In contrast, IL-2 weakly induced differentiation to CD8. Interestingly, Con A sup strongly induced differentiation to CD8 SP from the purified DP thymocytes prepared from C57BL/6 or LCMV TCRtg mice. In particular, it was found that thymocyte culture with Con A sup generated CD69+DP cells, and the CD69+DP differentiated to CD8 SP under the suspension culture with soluble factors. Thus, Con A sup or combinations of IL-2, IL-4 and IL-7 strongly induced differentiation of CD69+DP to CD8 SP, whereas individual cytokines did not. These results suggest that soluble factors like cytokines play an important role in the generation of SP thymocytes in the absence of thymic stromal cells, at least from a distinctive subpopulation like CD69+DP thymocytes, and perhaps from those of broader range when in conjunction with TCR/MHC interaction.
Authors:
Ki-Young Lee; Baik-Lin Seong; Kilhyoun Kim
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  70     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-10-07     Completed Date:  2003-03-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  410-21     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, College of Engineering, Yonesei University, Seoul, Korea. Khyounk@mm.ewha.ac.kr
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MeSH Terms
Descriptor/Qualifier:
Animals
Annexins / physiology
Antigens, CD4 / immunology*
Antigens, CD8 / immunology*
Apoptosis / physiology
Cell Differentiation / immunology*
Cytokines / physiology
Mice
Mice, Inbred C57BL
Thymus Gland / cytology*,  immunology*,  physiology
Chemical
Reg. No./Substance:
0/Annexins; 0/Antigens, CD4; 0/Antigens, CD8; 0/Cytokines

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