Document Detail


Soluble and cell-bound forms of steel factor activity play distinct roles in melanocyte precursor dispersal and survival on the lateral neural crest migration pathway.
MedLine Citation:
PMID:  7536655     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Trunk neural crest cells segregate from the neuroepithelium and enter a 'migration staging area' lateral to the embryonic neural tube. After some crest cells in the migration staging area have begun to migrate on a medial pathway, a subpopulation of crest-derived cells remaining in the migration staging area expresses mRNAs for the receptor tyrosine kinase, c-kit, and tyrosinase-related protein-2, both of which are characteristic of melanocyte precursors. These putative melanocyte precursors are subsequently observed on the lateral crest migration pathway between the dermatome and overlying epithelium, and then dispersed in nascent dermal mesenchyme. Melanocyte precursors transiently require the c-kit ligand, Steel factor for survival. Although Steel factor mRNA is transiently expressed in the dorsal dermatome before the onset of trunk neural crest cell dispersal on the lateral pathway, it is no longer produced by dermatomal cells when melanocyte precursors have dispersed in the dermal mesenchyme. To assess the role of Steel factor in migration of melanocyte precursors on the lateral pathway, we analyzed melanocyte precursor dispersal and fate on the lateral pathway of two different Sl mutants, Sl, a null allele, and Sld, which lacks cell surface-associated Steel factor but produces a soluble form. No melanocyte precursors were detected in the dermatome of embryos homozygous for the Sl allele or in W mutants that lack functional c-kit. In contrast, in embryos homozygous for the Sld allele, melanocyte precursors appeared on the lateral pathway, but subsequently disappear from the dermis. These results suggest that soluble Steel factor is required for melanocyte precursor dispersal on the lateral pathway, or for their initial survival in the migration staging area. In contrast, membrane-bound Steel factor appears to promote melanocyte precursor survival in the dermis.
Authors:
B Wehrle-Haller; J A Weston
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  121     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-05-24     Completed Date:  1995-05-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  731-42     Citation Subset:  IM    
Affiliation:
Institute of Neuroscience, University of Oregon, Eugene 97403.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement / physiology
Cell Survival / physiology
Central Nervous System / embryology*
Gene Expression
Genotype
Hematopoietic Cell Growth Factors / genetics,  physiology*
Immunohistochemistry
In Situ Hybridization
Melanocytes / cytology*
Mesoderm / cytology*,  physiology
Mice
Mice, Mutant Strains
Molecular Sequence Data
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins c-kit
RNA, Messenger / analysis
Receptor Protein-Tyrosine Kinases / genetics
Receptors, Colony-Stimulating Factor / genetics
Skin / embryology*
Stem Cell Factor
Grant Support
ID/Acronym/Agency:
DE-04316/DE/NIDCR NIH HHS; DE-05620/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Hematopoietic Cell Growth Factors; 0/Proto-Oncogene Proteins; 0/RNA, Messenger; 0/Receptors, Colony-Stimulating Factor; 0/Stem Cell Factor; EC 2.7.10.1/Proto-Oncogene Proteins c-kit; EC 2.7.10.1/Receptor Protein-Tyrosine Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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