| Soluble HLA-G is an independent factor for the prediction of pregnancy outcome after ART: a German multi-centre study. | |
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MedLine Citation:
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PMID: 20488801 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Soluble HLA-G (sHLA-G) has been suggested as a non-invasive marker for embryo selection to improve pregnancy rates after assisted reproduction technique (ART). Our study aimed at the identification of parameters influencing the detection of sHLA-G in embryo cultures (ECs) and at the prognostic relevance of sHLA-G in a multi-centre study. METHODS: In total 4212 EC from 2364 cycles were randomly collected from 29 German ART centres and analysed for sHLA-G by Luminex-based technology. RESULTS: Among test and culture conditions, only the cleavage stage of the embryo was identified as an independent factor for sHLA-G detection (P < 0.001). Overall, sHLA-G was significantly associated with pregnancy after ART [P < 0.001; odds ratio: 2.0 (95% CI: 1.7-2.4)], suggesting that sHLA-G testing might improve the pregnancy rate from 30 to 40%. Importantly, the sHLA-G status of embryos could be associated with pregnancy after single embryo transfer [P = 0.002; odds ratio: 3.3 (95% CI: 1.5-6.8)] doubling the probability of pregnancy rate to 26% after sHLA-G testing. The patient's age, number of transferred embryos, morphological grading [EXP(B): 4.3 (95% CI: 2.1-8.9)] of embryos and sHLA-G status [EXP(B): 2.3 (95% CI: 1.8-3.1)] were independent predictors of pregnancy, with the latter two being most powerful. CONCLUSIONS: This study provides significant evidence that the morphological scoring system is still the best strategy for the selection of embryos but that sHLA-G might be considered as a second parameter if a choice has to be made between embryos of morphologically equal quality. |
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Authors:
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V Rebmann; M Switala; I Eue; H Grosse-Wilde |
Publication Detail:
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Type: Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't Date: 2010-05-19 |
Journal Detail:
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Title: Human reproduction (Oxford, England) Volume: 25 ISSN: 1460-2350 ISO Abbreviation: Hum. Reprod. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-08-12 Completed Date: 2010-12-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8701199 Medline TA: Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 1691-8 Citation Subset: IM |
Affiliation:
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Institut für Transfusionsmedizin, Universitätsklinikum Essen, Virchowstrasse 179, 45147 Essen, Germany. vera.rebmann@uk-essen.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Markers
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metabolism Embryo Culture Techniques Embryo, Mammalian / metabolism* Embryonic Development Female Germany HLA Antigens / metabolism* Histocompatibility Antigens Class I / metabolism* Humans Predictive Value of Tests Pregnancy Pregnancy Rate* Reproductive Techniques, Assisted* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/HLA Antigens; 0/HLA-G antigen; 0/Histocompatibility Antigens Class I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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