Document Detail

Soluble HLA-G is an independent factor for the prediction of pregnancy outcome after ART: a German multi-centre study.
MedLine Citation:
PMID:  20488801     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Soluble HLA-G (sHLA-G) has been suggested as a non-invasive marker for embryo selection to improve pregnancy rates after assisted reproduction technique (ART). Our study aimed at the identification of parameters influencing the detection of sHLA-G in embryo cultures (ECs) and at the prognostic relevance of sHLA-G in a multi-centre study.
METHODS: In total 4212 EC from 2364 cycles were randomly collected from 29 German ART centres and analysed for sHLA-G by Luminex-based technology.
RESULTS: Among test and culture conditions, only the cleavage stage of the embryo was identified as an independent factor for sHLA-G detection (P < 0.001). Overall, sHLA-G was significantly associated with pregnancy after ART [P < 0.001; odds ratio: 2.0 (95% CI: 1.7-2.4)], suggesting that sHLA-G testing might improve the pregnancy rate from 30 to 40%. Importantly, the sHLA-G status of embryos could be associated with pregnancy after single embryo transfer [P = 0.002; odds ratio: 3.3 (95% CI: 1.5-6.8)] doubling the probability of pregnancy rate to 26% after sHLA-G testing. The patient's age, number of transferred embryos, morphological grading [EXP(B): 4.3 (95% CI: 2.1-8.9)] of embryos and sHLA-G status [EXP(B): 2.3 (95% CI: 1.8-3.1)] were independent predictors of pregnancy, with the latter two being most powerful.
CONCLUSIONS: This study provides significant evidence that the morphological scoring system is still the best strategy for the selection of embryos but that sHLA-G might be considered as a second parameter if a choice has to be made between embryos of morphologically equal quality.
V Rebmann; M Switala; I Eue; H Grosse-Wilde
Publication Detail:
Type:  Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't     Date:  2010-05-19
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  25     ISSN:  1460-2350     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-08-12     Completed Date:  2010-12-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  1691-8     Citation Subset:  IM    
Institut für Transfusionsmedizin, Universitätsklinikum Essen, Virchowstrasse 179, 45147 Essen, Germany.
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MeSH Terms
Biological Markers / metabolism
Embryo Culture Techniques
Embryo, Mammalian / metabolism*
Embryonic Development
HLA Antigens / metabolism*
Histocompatibility Antigens Class I / metabolism*
Predictive Value of Tests
Pregnancy Rate*
Reproductive Techniques, Assisted*
Reg. No./Substance:
0/Biological Markers; 0/HLA Antigens; 0/HLA-G antigen; 0/Histocompatibility Antigens Class I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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