Document Detail


Soluble Flt-1 and PlGF: new markers of early pregnancy loss?
MedLine Citation:
PMID:  21448460     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent data have indicated a relationship between placental oxygen and angiogenic protein levels in the first trimester of normal pregnancies. Our objective was to investigate if maternal serum levels of angiogenic factors Soluble vascular endothelial growth factor (VEGF) receptor 1 (sFlt-1), soluble Endoglin and placental growth factor (PlGF) are altered in women with symptoms of threatened miscarriage (TM) and if they are predictive of a subsequent miscarriage. Blood samples were collected at 6-10 weeks from women presenting with TM (n = 40), from asymptomatic controls (n = 32) and from non- pregnant women in their luteal phase (n = 14). All samples were assayed for serum level of sFLT-1, PlGF, sEndoglin and HSP70 using commercial ELISAs. Samples were analysed retrospectively on the basis of pregnancy outcome. TM group included 21 women with a normal pregnancy outcome and 19 with subsequent complete miscarriage. The latter subgroup had significantly lower mean maternal serum (MS) sFlt-1 (83%, P<0.001) and PlGF (44%, P<0.001) compared to those with a normal pregnancy outcome. Asymptomatic control pregnant women had similar MS levels of sFlt-1 and PlGF compared to the TM patients with a normal outcome. The mean MS sFlt-1 (>10 fold) and MS PlGF (∼2 fold) levels were significantly (P<0.001) higher in control pregnant women compared to the non-pregnant group in the luteal phase of the menstrual cycle. Soluble Endoglin was not altered in the normal pregnant women compared to non pregnant women, although lower in the TM subgroup with a subsequent miscarriage (∼25%, P<0.001) compared to TM with a live birth. There was no significant difference in the mean MS HSP 70 levels between the different groups. This study shows that sFlt1 and PlGF MS levels are increased by several folds in early pregnancy and that MS sFlt-1 and MS PlGF are markedly decreased in threatened miscarriage patients who subsequently have a miscarriage suggesting these proteins are sensitive predictive markers of subsequent pregnancy loss.
Authors:
Shanthi Muttukrishna; Michelle Swer; Sangeeta Suri; Amna Jamil; Jean Calleja-Agius; Subrata Gangooly; Helen Ludlow; Davor Jurkovic; Eric Jauniaux
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-03-23
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-03-30     Completed Date:  2011-07-05     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e18041     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, Anu Research Centre, University College Cork, Cork University Maternity Hospital, Wilton, Cork, Republic of Ireland. S.Muttukrishna@ucc.ie
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MeSH Terms
Descriptor/Qualifier:
Adult
Antigens, CD / blood
Biological Markers / blood
Demography
Embryo Loss / blood*
Female
HSP70 Heat-Shock Proteins / blood
Humans
Membrane Proteins / blood*
Pregnancy
Receptors, Cell Surface / blood
Solubility
Vascular Endothelial Growth Factor Receptor-1 / blood*
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Biological Markers; 0/ENG protein, human; 0/HSP70 Heat-Shock Proteins; 0/Membrane Proteins; 0/PIGF protein, human; 0/Receptors, Cell Surface; EC 2.7.10.1/FLT1 protein, human; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-1
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