Document Detail


Solubilization and reconstitution of an amiloride-inhibited sodium transporter from rabbit kidney medulla.
MedLine Citation:
PMID:  7093216     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An octyl glucoside extract has been formed from rabbit kidney medulla microsomes from which reconstituted proteoliposomes can be formed by lipid addition and dialysis to remove detergent. These proteoliposomes are capable of amiloride-inhibited 22Na+ transport. The amiloride-inhibited Na+ transport process is complete within 10 min and directly proportional to the vesicle protein concentration. Sodium accumulation by the proteoliposomes has been proven to represent transport by the demonstration that all Na+ taken up by the vesicles can be removed by the ionophore nigericin. The process has been shown to be specific for amiloride by the demonstration that the effect of amiloride on Na+ transport could not be reproduced by the similar compound sulfaguanidine nor by pyrazine, 2-pyrazinecarboxamide, 2-pyrazinecarboxylate, or 3-amino-2-pyrazinecarboxylate. The relationship between Na+ uptake into proteoliposomes and Na+ concentration was similar to the relationship between Na+ uptake and concentration observed with medulla microsomes. The concentration of amiloride required for half-maximal inhibition of Na+ uptake into either proteoliposomes or medulla microsomes was also the same. The evidence seems clear that the protein responsible for amiloride-inhibited Na+ transport into rabbit kidney medulla microsomes has been extracted from the membranes and incorporated into purified lipid vesicles.
Authors:
E F LaBelle; S O Lee
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  21     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1982 May 
Date Detail:
Created Date:  1982-09-24     Completed Date:  1982-09-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2693-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amiloride / pharmacology*
Biological Transport, Active / drug effects
Kidney Medulla / analysis*
Liposomes / metabolism
Microsomes / analysis
Nigericin / pharmacology
Pyrazines / pharmacology*
Sodium / antagonists & inhibitors*
Solubility
Grant Support
ID/Acronym/Agency:
AM-25244/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Liposomes; 0/Pyrazines; 2609-46-3/Amiloride; 28380-24-7/Nigericin; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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