Document Detail


Solubilization and partial properties of receptor substance for bacteriophage alpha 2 induced from Clostridium botulinum type A 190L.
MedLine Citation:
PMID:  3913846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacteriophage alpha 2, one of the two inducible phages from Clostridium botulinum type A 190L, had a latent period of 55 min and an average burst size of 75 in C. botulinum type A Hall used as the host bacterium. The phage particles were adsorbed on the cell walls extracted with hot trichloroacetic acid (TCA-walls). The receptor substance for the phage was solubilized from the TCA-walls with Achromopeptidase and fractionated by gel filtration on Sephadex G-150. The fraction having the highest level of receptor activity for the phage contained large amounts of muramic acid and glucosamine. Both authentic muramic acid and glucosamine significantly inactivated the phage, whereas glucose, galactose, L-and D-alanine, diaminopimeric acid, or D-glutamic acid did not exhibit similar activity. There results strongly suggest that the receptor site for phage alpha 2 is closely associated with glycan moieties of the cell wall peptidoglycan.
Authors:
K Takumi; A Takeoka; T Kinouchi; T Kawata
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbiology and immunology     Volume:  29     ISSN:  0385-5600     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  1985  
Date Detail:
Created Date:  1986-05-22     Completed Date:  1986-05-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1185-95     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacteriophages / analysis*,  isolation & purification,  ultrastructure
Cell Wall / analysis*
Chromatography, Gel
Clostridium botulinum / analysis*
Endopeptidases
Glucosamine / analysis
Microscopy, Electron
Muramic Acids / analysis
Receptors, Virus / analysis*
Serine Endopeptidases*
Trichloroacetic Acid
Chemical
Reg. No./Substance:
0/Muramic Acids; 0/Receptors, Virus; 3416-24-8/Glucosamine; 76-03-9/Trichloroacetic Acid; EC 3.4.-/Endopeptidases; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.50/lysyl endopeptidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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