Document Detail

Solubilization of hydroxyapatite crystals by murine bone cells, macrophages and fibroblasts.
MedLine Citation:
PMID:  2611305     Owner:  NLM     Status:  MEDLINE    
Calcium phosphate ceramic is widely used as implant material. It is made up of hydroxyapatite, beta-tricalcium phosphate or various combinations of both. In the present study, we use an in vitro model to examine the role of cell-mediated resorption of calcium phosphate ceramic implant material. We compare the abilities of two sequential enzymatic released populations of bone cells from murine calvaria (Population II and Population V), macrophages and dermal fibroblasts to solubilize 45Ca-labelled hydroxyapatite crystals. These crystals were incubated with each of the cell types for 24 h in the presence or absence of parathyroid hormone, prostaglandin E2, calcitonin, and 1,25-dihydroxyvitamin D3. The amount of cell-mediated hydroxyapatite solubilization was determined by measuring the radioactivity in an aliquot of the supernatant after centrifugation. Using dermal fibroblasts as a baseline, relative abilities of macrophages, Population II and Population V to degrade crystals were 10.5, 5 and 2 times that of fibroblasts. Crystal-cell contact was required. While none of the bone resorption agents tested had any effect on this process, crystal dissolution by bone cells was inhibited by two lysosomotropic agents, NH4Cl and chloroquine.
C H Kwong; W B Burns; H S Cheung
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biomaterials     Volume:  10     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1990-03-07     Completed Date:  1990-03-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  579-84     Citation Subset:  IM    
Department of Medicine, Medical College of Wisconsin, Milwaukee 53226.
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MeSH Terms
Ammonium Chloride / pharmacology
Bone and Bones / cytology*,  drug effects,  metabolism
Calcium Phosphates / metabolism*
Chloroquine / pharmacology
Fibroblasts / drug effects,  metabolism
Hydroxyapatites / metabolism*
Macrophages / drug effects,  metabolism*
Prostheses and Implants
Skin / cytology
Grant Support
Reg. No./Substance:
0/Calcium Phosphates; 0/Hydroxyapatites; 12125-02-9/Ammonium Chloride; 54-05-7/Chloroquine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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