Document Detail

Solubility of tissue hydroxyproline in experimental intestinal anastomoses.
MedLine Citation:
PMID:  4043344     Owner:  NLM     Status:  MEDLINE    
Salt and acid solubility of collagen are thought to reflect its degree of crosslinking. In order to examine postoperative changes in crosslinking of intestinal collagen, which are of importance to the stability of the intestinal wall, we have investigated the solubility of hydroxyproline in rabbit ileum and colon, both in unwounded intestine and after construction of an anastomosis. Solubility in salt and dilute acid was increased by a sonication procedure. This way, 9% of total hydroxyproline in the unwounded colonic wall was salt soluble and 65% acid soluble. A similar distribution was observed in ileum. Three days after operation the salt-soluble fraction was significantly elevated in samples from the anastomotic area. In colon, this increase also persisted 7 days postoperatively. Comparison of anastomotic samples collected 3 and 7 days after surgery shows a significant decrease in the acid-soluble and a significant increase in the solid fraction. This phenomenon occurs both in ileum and colon. These results indicate that during the first days after operation the integrity of the intestinal wall is weakened not only by a loss of collagen, but also by a changed solubility of the remaining collagen.
T Hendriks; W L Hesp; A A Klompmakers; E J Lubbers; H H de Boer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental and molecular pathology     Volume:  43     ISSN:  0014-4800     ISO Abbreviation:  Exp. Mol. Pathol.     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1985-10-25     Completed Date:  1985-10-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370711     Medline TA:  Exp Mol Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  253-9     Citation Subset:  IM    
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MeSH Terms
Colon / metabolism,  surgery*
Hydroxyproline / metabolism*
Ileum / metabolism,  surgery*
Time Factors
Reg. No./Substance:

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