| Solid tumor cells express functional "tethered ligand" thrombin receptor. | |
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MedLine Citation:
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PMID: 7834643 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous work demonstrated that alpha-thrombin promoted tumor cell adhesion to endothelium and extracellular matrix as well as enhanced the metastatic capacity of tumor cells. This study was initiated to investigate whether the thrombin effect on tumor cells is mediated through the "tethered ligand" thrombin receptor. RT-PCR analysis using primers based on the human thrombin receptors detected mRNA in human colon adenocarcinoma cells (clone A), whose authenticity was confirmed by Southern hybridization. The presence of thrombin receptor mRNA in rat (W256 carcinosarcoma) and mouse (melanoma) tumor cells was demonstrated by RT-PCR/Southern blotting using species-specific PCR primers. Sequencing of the PCR fragment of clone A cells revealed complete homology with the reported human cDNA sequence. Subsequently, tumor cells derived from three species, i.e., human, rat, and mouse, were found to express the thrombin receptor protein as revealed by immunoblotting using ligand peptide-derived mAb ATAP138, whose reactivity towards the M(r) approximately 66,000, potential thrombin receptor was blocked by preincubating the antibody with the immunogen peptide SFLLRNPNDKYEPF (TRP 14). Finally, peptides TRP 14 and TRP 7 (SFLLRNP), but not TRP 5 (FLLRN), were found to mimic alpha-thrombin in stimulating tumor cell adhesion to fibronectin, suggesting that the thrombin receptors expressed on solid tumor cells are biologically functional. |
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Authors:
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M Z Wojtukiewicz; D G Tang; E Ben-Josef; C Renaud; D A Walz; K V Honn |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer research Volume: 55 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1995 Feb |
Date Detail:
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Created Date: 1995-02-28 Completed Date: 1995-02-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 698-704 Citation Subset: IM |
Affiliation:
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Department of Radiation Oncology, Wayne State University, Detroit, Michigan 48202. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/S76625 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Blotting, Western Cell Adhesion Cell Membrane / metabolism DNA Primers DNA, Neoplasm / analysis, metabolism Fibronectins Humans Mice Molecular Sequence Data Neoplasms / metabolism* Neoplasms, Experimental / metabolism* Polymerase Chain Reaction / methods Rats Receptors, Thrombin / analysis, biosynthesis* Species Specificity |
| Grant Support | |
ID/Acronym/Agency:
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CA 47115/CA/NCI NIH HHS; HL 27073/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Neoplasm; 0/Fibronectins; 0/Receptors, Thrombin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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