Document Detail


Solid-phase sequence scanning for drug resistance detection in tuberculosis.
MedLine Citation:
PMID:  10208797     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DNA chip arrays hold considerable promise for diagnostic sequencing of polymerase chain reaction (PCR) products. To date, however, arrays have been relatively expensive, complex to use and difficult to interpret, preventing their adaptation to the clinical lab. A moderate density array method has been developed that enables efficient, easy-to-interpret and robust solid-phase PCR product sequencing. Here, the results of Mycobacterium tuberculosis rifampin resistance mutation detection by primer-extension-based sequence scanning of the rpo B gene of M. tuberculosis are presented. Rifampin resistant clinical isolates were identified in as little as 1 h post PCR amplification with visual results detection.
Authors:
S R Head; K Parikh; Y H Rogers; W Bishai; P Goelet; M T Boyce-Jacino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular probes     Volume:  13     ISSN:  0890-8508     ISO Abbreviation:  Mol. Cell. Probes     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-07-06     Completed Date:  1999-07-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8709751     Medline TA:  Mol Cell Probes     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  81-7     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
Alpha Center, Orchid Biocomputer, Inc., Hopkins Bayview Research Campus, 5210 Eastern Avenue, Baltimore, Maryland, 21224, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics*
Codon / genetics
DNA Mutational Analysis / methods*
DNA Primers
DNA, Bacterial / genetics*
Drug Resistance, Microbial / genetics*
Genes, Bacterial
Genotype
Humans
Image Processing, Computer-Assisted
Mycobacterium tuberculosis / drug effects,  genetics*,  isolation & purification
Oligonucleotide Array Sequence Analysis / methods*
Plant Proteins / genetics*
Polymerase Chain Reaction
Rifampin / pharmacology*
Sequence Analysis, DNA
Software
Time Factors
Tuberculosis / drug therapy,  microbiology*
Grant Support
ID/Acronym/Agency:
R29-AI36973/AI/NIAID NIH HHS; R43 AI39861-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Codon; 0/DNA Primers; 0/DNA, Bacterial; 0/Plant Proteins; 0/RpoB protein, Oryza sativa; 13292-46-1/Rifampin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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