Document Detail


Solid phase radioimmunoassay using labelled antibodies: a conceptual framework for designing assays.
MedLine Citation:
PMID:  833431     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple theoretical model for the antigen-antibody reaction is presented ans used to evaluate the optimum conditions for designing solid phase radioimmunoassay (RIA) using labelled antibodies. Both theoretical and experimental data are presented, using a wide variety of antigens and their corresponding antibodies. The types of RIA is described include the direct, the indirect, sandwich assays for detecting either antigen or antibody. The experimental results confirm in a semiquantitative manner that the greatest sensitivity of the RIA is achieved when the smallest amount of labelled antibody is used, that whenever possible the antigen/antibody ratio should be greater than unity (greater than 1), and that the formation of the antigen-antibody comples is dependent on the mass action effect.
Authors:
J Kalmakoff; A J Parkinson; A M Crawford; B R Williams
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  14     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1977  
Date Detail:
Created Date:  1977-03-15     Completed Date:  1977-03-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  73-84     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies* / analysis
Antigen-Antibody Reactions
Antigens / analysis
Humans
Immunoglobulin G / metabolism
Iodine Radioisotopes
Models, Biological
Radioimmunoassay
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens; 0/Immunoglobulin G; 0/Iodine Radioisotopes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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