Document Detail

Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies.
MedLine Citation:
PMID:  965475     Owner:  NLM     Status:  MEDLINE    
A solid-phase radioimmunoassay method has been developed for the detection of rubella virus-specific immunoglobulin G (IgG) and IgM antibodies in human serum specimens. Purified rubella virus was adsorbed onto polystyrene balls, and antibodies that attached to the virus-treated balls were detected by subsequent binding of 125I-labeled anti-human gamma or anti-human mu immunoglobulins. A total of 77 serum specimens were tested. Binding ratios between positive and negative sera were as high as 22 in the IgG assay but rarely exceeded 3 in the IgM assay. The sensitivity of the IgG assay was found to be 16 to 256 times higher than that of the rubella virus hemagglutination inhibition test. The IgG radioimmunoassay can be readily adopted for routine diagnostic use. The IgM radioimmunoassay, however, due to its lower sensitivity, must be modified before being routinely applied.
K O Kalimo; O H Meurman; P E Halonen; B R Ziola; M K Viljanen; K Granfors; P Toivanen
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  4     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1976 Aug 
Date Detail:
Created Date:  1976-12-01     Completed Date:  1976-12-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  117-23     Citation Subset:  IM    
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MeSH Terms
Antibodies, Viral / analysis*
Complement Fixation Tests
Diagnosis, Differential
Evaluation Studies as Topic
Hemagglutination Inhibition Tests
Immunoglobulin G / analysis*
Immunoglobulin M / analysis*
Iodine Radioisotopes / diagnostic use
Radioimmunoassay / methods*
Rubella / diagnosis
Rubella virus / immunology*
Reg. No./Substance:
0/Antibodies, Viral; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/Iodine Radioisotopes; 0/Polystyrenes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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