Document Detail


Solid-phase microextraction in the determination of methadone in human saliva by gas chromatography-mass spectrometry.
MedLine Citation:
PMID:  10732946     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Solid-phase microextraction (SPME) with a 100-microm polydimethylsiloxane film fiber was applied to the determination of methadone and 2-ethylidine-3,3-diphenylpyrrolidine (EDDP) by GC-MS in human saliva and compared with liquid-liquid extraction. A shorter extraction time of 30 min with the fiber was obtained, speeding up the total analysis time. Linearity was found for SPME from 0.05 to 2.0 microg/mL (r = 0.9976 for methadone; r = 0.9988 for EDDP) with precision between 0.7 and 4.3% for saliva spiked with 0.2 and 1.5 microg/mL of methadone and EDDP. The limit of detection using SPME was 0.04 microg/mL for methadone and 0.008 microg/mL for EDDP. Analytical recoveries of SPME and liquid-liquid extraction ranged from 98.8 to 103.6%. The use of deuterated internal standard by both methods have yielded comparable results. Thus, the SPME method is highly accurate, precise, and useful for determination of methadone and EDDP in saliva.
Authors:
A C dos Santos Lucas; A Bermejo; P Fernández; M J Tabernero
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of analytical toxicology     Volume:  24     ISSN:  0146-4760     ISO Abbreviation:  J Anal Toxicol     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-04-11     Completed Date:  2000-04-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705085     Medline TA:  J Anal Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  93-6     Citation Subset:  IM    
Affiliation:
Department of Clinical Analysis and Toxicology, Faculty of Pharmacy, University of Amazonas, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Dimethylpolysiloxanes / chemistry
Gas Chromatography-Mass Spectrometry / methods*
Humans
Methadone / analysis*,  isolation & purification
Pyrrolidines / analysis
Reproducibility of Results
Saliva / chemistry*
Silicones / chemistry
Substance Abuse Detection / methods
Chemical
Reg. No./Substance:
0/Dimethylpolysiloxanes; 0/Pyrrolidines; 0/Silicones; 30223-73-5/2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine; 63148-62-9/baysilon; 76-99-3/Methadone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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