| Solid-phase extraction of ursolic acid from herb using beta-cyclodextrin-based molecularly imprinted microspheres. | |
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MedLine Citation:
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PMID: 18844208 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new method was employed to solid-phase extract ursolic acid from Ilex kudingcha C. J. Tseng using molecularly imprinted microspheres (MIMs) as the sorbent. Using a surface molecular imprinting technique, MIMs for ursolic acid were prepared with bonded beta-CD and acrylamide in combination based on functionalized poly(glycidyl methacrylate) microspheres (F-PGMA). Compared with non-MIMs (NIMs), MIMs showed high adsorption capacity, significant selectivity, and good site accessibility for ursolic acid. The maximum static adsorption capacities of the MIMs and NIMs for ursolic acid were 42.5 and 4.9 micromol/g, respectively. Chromatographic analysis shows that ursolic acid and oleanolic acid could be separated well when MIMs were used as the stationary phase of HPLC. The conditions of molecularly imprinted SPE (MISPE) of ursolic acid from the herb extract were optimized using different concentrations of ethanol solutions as loading, washing, and eluting solutions. The successful extraction of ursolic acid by MIMs provided a possible innovative approach to separate ursolic acid from herb. |
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Authors:
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Hanmao Liu; Chunhai Liu; Xiaojing Yang; Songjun Zeng; Yuanqin Xiong; Weijian Xu |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of separation science Volume: 31 ISSN: 1615-9314 ISO Abbreviation: J Sep Sci Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-11-03 Completed Date: 2009-01-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088554 Medline TA: J Sep Sci Country: Germany |
Other Details:
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Languages: eng Pagination: 3573-80 Citation Subset: IM |
Affiliation:
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College of Chemistry and Chemical Engineering, Hunan University, Changsha, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Antineoplastic Agents, Phytogenic / chemistry, isolation & purification* Chromatography, High Pressure Liquid / methods Hydrogen-Ion Concentration Ilex / chemistry* Microspheres* Molecular Imprinting* Molecular Structure Oleanolic Acid / chemistry, isolation & purification Solid Phase Extraction* / instrumentation, methods Triterpenes / chemistry, isolation & purification* beta-Cyclodextrins / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/Triterpenes; 0/beta-Cyclodextrins; 508-02-1/Oleanolic Acid; 7585-39-9/betadex; 77-52-1/ursolic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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