Document Detail


Solid-Supported NOTA and DOTA Chelators Useful for the Synthesis of 3'-Radiometallated Oligonucleotides.
MedLine Citation:
PMID:  22871148     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Esterified precursors of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA; 18) and 1,4,7-triazacyclononane-1,4,7-trisacetic acid (NOTA; 17,19) ligands bearing a dimethoxytritylated hydroxyl side arm were prepared and immobilized via an ester linkage to long chain alkyl amine derivatized controlled pore glass (LCAA-CPG). Oligonucleotide chains were then assembled on the hydroxyl function and conjugates were released and deprotected by a two-step cleavage with aqueous alkali and ammonia. The 3'-DOTA and 3´-NOTA conjugated oligonucleotides were converted to 68Ga chelates by a brief treatment with [68Ga]Cl3 at elevated temperature. Applicability of the conjugates for in vivo imaging with positron emission tomography (PET) was verified.
Authors:
Pasi Virta; Anu Kiviniemi; Joonas Mäkelä; Jussi Mäkilä; Tiina Saanijoki; Heidi Liljenbäck; Paivi Poijarvi-Virta; Harri Lonnberg; Tiina Laitala-Leinonen; Anne Roivainen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-8
Journal Detail:
Title:  Bioconjugate chemistry     Volume:  -     ISSN:  1520-4812     ISO Abbreviation:  Bioconjug. Chem.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010319     Medline TA:  Bioconjug Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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