Document Detail

Soleus adaptation to combined exercise and heat acclimation: physiogenomic aspects.
MedLine Citation:
PMID:  19996992     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: Adaptation to heat (acclimation [ACC]) and exercise training (EX) require global changes at all levels of body organization to enhance muscle performance. In this investigation, we combined these stressors and examined physiological and genomic aspects of adaptation in skeletal muscle (soleus). METHODS: Rats were divided into four groups: C (controls), ACC--acclimated to heat only at 34 degrees C, EX--aerobic exercise on a treadmill at 24 degrees C, and EXAC--acclimated to combined heat and aerobic training. The ACC period was 30 d. Isometric force generation was measured using isolated muscle preparations stimulated at 1-100 Hz, allowing assessment of muscle endurance. Global genomic responses of homeostatic genes were detected using a complementary DNA (cDNA) Atlas array (Rat 1.2; Clontech Laboratory, Palo Alto, CA). RESULTS: Significantly elevated force generation (P < 0.05) was only found in the EXAC group along with a marked decrease in relaxation velocity. Both heat-treated groups (ACC and EXAC) demonstrated less of a drop in power at stimulation frequencies above the highest force generation (P < 0.05). Gene reprogramming was noted in all treatment groups with stressor-specific dynamic profiles. Improved force generation in the EXAC soleus coincided with significant up-regulation in expression levels of genes encoding sarcoplasmic Ca2+-transporting proteins (SERCA2 and inositol triphosphate receptor), glycolysis rate-limiting enzyme (phosphofructokinase), mitochondrial lipid metabolism (CPTII), and stress proteins with antiapoptotic or apoptotic activity. CONCLUSIONS: Our data suggest that EXAC-specific gene up-regulation and cross talk between genes assigned to their gene ontology categories (transport, metabolism, and stress) differ in abundance and/or expression level (compared with other treatment groups) and contributed to the physiological advantage demonstrated by the EXAC soleus.
Einat Kodesh; Michal Horowitz
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medicine and science in sports and exercise     Volume:  42     ISSN:  1530-0315     ISO Abbreviation:  Med Sci Sports Exerc     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-20     Completed Date:  2010-08-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8005433     Medline TA:  Med Sci Sports Exerc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  943-52     Citation Subset:  IM; S    
Laboratory of Environmental Physiology, Faculty of Dental Medicine, The Hebrew University, Jerusalem, Israel.
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MeSH Terms
Cytoprotection / genetics
Gene Expression*
Hot Temperature*
Isometric Contraction
Muscle, Skeletal / metabolism*
Physical Conditioning, Animal / methods,  physiology*

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