| Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium. | |
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MedLine Citation:
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PMID: 10361493 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An acid-stable soybean-peroxidase biosensor was developed by immobilizing the enzyme in a sol-gel thin film. Methylene blue was used as a mediator because of its high electron-transfer efficiency. The sol-gel thin film and enzyme membrane were characterized by FT-IR, and the effects of pH, operating potential, and temperature were explored for optimum analytical performance by using the amperometric method. The H2O2 sensor exhibited a fast response (5 s), high sensitivity (27.5 microA/mM), as well as good thermostability and long-term stability. In addition, the performance of the biosensor was investigated using flow-injection analysis (FIA). |
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Authors:
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B Wang; B Li; Z Wang; G Xu; Q Wang; S Dong |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical chemistry Volume: 71 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 1999 May |
Date Detail:
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Created Date: 1999-07-27 Completed Date: 1999-07-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1935-9 Citation Subset: IM |
Affiliation:
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Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin, PRC. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Enzymes, Immobilized
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chemistry* Hydrogen Peroxide / analysis* Hydrogen-Ion Concentration Membranes, Artificial Oxidants / analysis* Peroxidases / chemistry* Soybeans / enzymology* |
| Chemical | |
Reg. No./Substance:
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0/Enzymes, Immobilized; 0/Membranes, Artificial; 0/Oxidants; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.-/Peroxidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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