Document Detail


Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium.
MedLine Citation:
PMID:  10361493     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An acid-stable soybean-peroxidase biosensor was developed by immobilizing the enzyme in a sol-gel thin film. Methylene blue was used as a mediator because of its high electron-transfer efficiency. The sol-gel thin film and enzyme membrane were characterized by FT-IR, and the effects of pH, operating potential, and temperature were explored for optimum analytical performance by using the amperometric method. The H2O2 sensor exhibited a fast response (5 s), high sensitivity (27.5 microA/mM), as well as good thermostability and long-term stability. In addition, the performance of the biosensor was investigated using flow-injection analysis (FIA).
Authors:
B Wang; B Li; Z Wang; G Xu; Q Wang; S Dong
Related Documents :
6772093 - L-lysine epsilon-aminotransferase involved in cephamycin c synthesis in streptomyces la...
17354173 - Synthesis and spectroscopic characterization of cannabinolic acid.
18992773 - Antiviral activity of quercetin 7-rhamnoside against porcine epidemic diarrhea virus.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  71     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-07-27     Completed Date:  1999-07-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1935-9     Citation Subset:  IM    
Affiliation:
Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin, PRC.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Enzymes, Immobilized / chemistry*
Hydrogen Peroxide / analysis*
Hydrogen-Ion Concentration
Membranes, Artificial
Oxidants / analysis*
Peroxidases / chemistry*
Soybeans / enzymology*
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Membranes, Artificial; 0/Oxidants; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.-/Peroxidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Uricase-catalyzed oxidation of uric acid using an artificial electron acceptor and fabrication of am...
Next Document:  Electrochemical sensor for measurement of urea and creatinine in serum based on ac impedance measure...