Document Detail


Sol-gel derived amperometric nitric oxide microsensor.
MedLine Citation:
PMID:  15924380     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An amperometric sol-gel derived nitric oxide microsensor is described. Several silicon-based xerogel membranes are evaluated to identify the optimum composition for maximizing NO permeability while providing sufficient selectivity for NO in the presence of common interfering species. Xerogel permeability and selectivity are further manipulated as a function of reaction/processing conditions. In addition, the effects of incorporating Nafion into the xerogel matrix on sensor performance and the stability of the ensuing xerogel/Nafion hybrid film are evaluated. The optimal permselective membrane is achieved by catalyzing polycondensation of the xerogel composed of methyltrimethoxysilane and (aminoethylaminomethyl)phenethyltrimethoxysilane and Nafion with NO gas. The resulting NO microsensor exhibits a sensitivity of 0.17 +/-0.02 pA/nM (from 25 to 800 nM, r = 0.9991), detection limit of 25 nM (S/N = 3), response time of 9 s (t(95%), a NO concentration change from 400 to 500 nM), selectivity (log K(NOj) amp) of -5.8, <-6, <-6, and <-6 for j = nitrite, ascorbic acid, uric acid, and acetaminophen, and a lifetime of 8 d (82% of initial sensitivity without serious deterioration in selectivity).
Authors:
Jae Ho Shin; Stephen W Weinman; Mark H Schoenfisch
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Analytical chemistry     Volume:  77     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-31     Completed Date:  2007-04-02     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3494-501     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
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MeSH Terms
Descriptor/Qualifier:
Electrochemistry
Fluorocarbon Polymers / chemistry*
Gels / chemistry*
Membranes, Artificial*
Microelectrodes
Nitric Oxide / analysis*,  chemistry
Sensitivity and Specificity
Silicon / chemistry
Surface Properties
Time Factors
Grant Support
ID/Acronym/Agency:
EB000708/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Fluorocarbon Polymers; 0/Gels; 0/Membranes, Artificial; 10102-43-9/Nitric Oxide; 39464-59-0/perfluorosulfonic acid; 7440-21-3/Silicon

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