| Soil amoebae rapidly change bacterial community composition in the rhizosphere of Arabidopsis thaliana. | |
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MedLine Citation:
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PMID: 19242534 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We constructed an experimental model system to study the effects of grazing by a common soil amoeba, Acanthamoeba castellanii, on the composition of bacterial communities in the rhizosphere of Arabidopsis thaliana. Amoebae showed distinct grazing preferences for specific bacterial taxa, which were rapidly replaced by grazing tolerant taxa in a highly reproducible way. The relative proportion of active bacteria increased although bacterial abundance was strongly decreased by amoebae. Specific bacterial taxa had disappeared already two days after inoculation of amoebae. The decrease in numbers was most pronounced in Betaproteobacteria and Firmicutes. In contrast, Actinobacteria, Nitrospira, Verrucomicrobia and Planctomycetes increased. Although other groups, such as betaproteobacterial ammonia oxidizers and Gammaproteobacteria did not change in abundance, denaturing gradient gel electrophoresis with specific primers for pseudomonads (Gammaproteobacteria) revealed both specific changes in community composition as well as shifts in functional genes (gacA) involved in bacterial defence responses. The resulting positive feedback on plant growth in the amoeba treatment confirms that bacterial grazers play a dominant role in structuring bacteria-plant interactions. This is the first detailed study documenting how rapidly protozoan grazers induce shifts in rhizosphere bacterial community composition. |
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Authors:
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Katja Rosenberg; Joanne Bertaux; Kristin Krome; Anton Hartmann; Stefan Scheu; Michael Bonkowski |
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Publication Detail:
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Type: Journal Article Date: 2009-02-26 |
Journal Detail:
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Title: The ISME journal Volume: 3 ISSN: 1751-7370 ISO Abbreviation: ISME J Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-05-19 Completed Date: 2009-06-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101301086 Medline TA: ISME J Country: England |
Other Details:
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Languages: eng Pagination: 675-84 Citation Subset: IM |
Affiliation:
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Technische Universität Darmstadt, Institut für Zoologie, Darmstadt, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acanthamoeba castellanii
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physiology* Animals Arabidopsis / growth & development, microbiology* Bacteria / classification*, genetics*, isolation & purification Biodiversity* DNA Fingerprinting DNA, Bacterial / genetics DNA, Ribosomal / genetics Electrophoresis, Polyacrylamide Gel In Situ Hybridization, Fluorescence Nucleic Acid Denaturation Plant Roots / microbiology* RNA, Ribosomal, 16S / genetics Soil Microbiology* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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