Document Detail


Soil amoebae rapidly change bacterial community composition in the rhizosphere of Arabidopsis thaliana.
MedLine Citation:
PMID:  19242534     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We constructed an experimental model system to study the effects of grazing by a common soil amoeba, Acanthamoeba castellanii, on the composition of bacterial communities in the rhizosphere of Arabidopsis thaliana. Amoebae showed distinct grazing preferences for specific bacterial taxa, which were rapidly replaced by grazing tolerant taxa in a highly reproducible way. The relative proportion of active bacteria increased although bacterial abundance was strongly decreased by amoebae. Specific bacterial taxa had disappeared already two days after inoculation of amoebae. The decrease in numbers was most pronounced in Betaproteobacteria and Firmicutes. In contrast, Actinobacteria, Nitrospira, Verrucomicrobia and Planctomycetes increased. Although other groups, such as betaproteobacterial ammonia oxidizers and Gammaproteobacteria did not change in abundance, denaturing gradient gel electrophoresis with specific primers for pseudomonads (Gammaproteobacteria) revealed both specific changes in community composition as well as shifts in functional genes (gacA) involved in bacterial defence responses. The resulting positive feedback on plant growth in the amoeba treatment confirms that bacterial grazers play a dominant role in structuring bacteria-plant interactions. This is the first detailed study documenting how rapidly protozoan grazers induce shifts in rhizosphere bacterial community composition.
Authors:
Katja Rosenberg; Joanne Bertaux; Kristin Krome; Anton Hartmann; Stefan Scheu; Michael Bonkowski
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Publication Detail:
Type:  Journal Article     Date:  2009-02-26
Journal Detail:
Title:  The ISME journal     Volume:  3     ISSN:  1751-7370     ISO Abbreviation:  ISME J     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-19     Completed Date:  2009-06-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101301086     Medline TA:  ISME J     Country:  England    
Other Details:
Languages:  eng     Pagination:  675-84     Citation Subset:  IM    
Affiliation:
Technische Universität Darmstadt, Institut für Zoologie, Darmstadt, Germany.
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MeSH Terms
Descriptor/Qualifier:
Acanthamoeba castellanii / physiology*
Animals
Arabidopsis / growth & development,  microbiology*
Bacteria / classification*,  genetics*,  isolation & purification
Biodiversity*
DNA Fingerprinting
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Electrophoresis, Polyacrylamide Gel
In Situ Hybridization, Fluorescence
Nucleic Acid Denaturation
Plant Roots / microbiology*
RNA, Ribosomal, 16S / genetics
Soil Microbiology*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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