Document Detail


Soft X-ray microscopy analysis of cell volume and hemoglobin content in erythrocytes infected with asexual and sexual stages of Plasmodium falciparum.
MedLine Citation:
PMID:  21945653     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plasmodium falciparum, the most virulent agent of human malaria, undergoes both asexual cycling and sexual differentiation inside erythrocytes. As the intraerythrocytic parasite develops it increases in size and alters the permeability of the host cell plasma membrane. An intriguing question is: how is the integrity of the host erythrocyte maintained during the intraerythrocytic cycle? We have used water window cryo X-ray tomography to determine cell morphology and hemoglobin content at different stages of asexual and sexual differentiation. The cryo stabilization preserves native structure permitting accurate analyses of parasite and host cell volumes. Absorption of soft X-rays by protein adheres to Beer-Lambert's law permitting quantitation of the concentration of hemoglobin in the host cell compartment. During asexual development the volume of the parasite reaches about 50% of the uninfected erythrocyte volume but the infected erythrocyte volume remains relatively constant. The total hemoglobin content gradually decreases during the 48h cycle but its concentration remains constant until early trophozoite stage, decreases by 25%, then remains constant again until just prior to rupture. During early sexual development the gametocyte has a similar morphology to a trophozoite but then undergoes a dramatic shape change. Our cryo X-ray tomography analysis reveals that about 70% of the host cell hemoglobin is taken up and digested during gametocyte development and the parasite eventually occupies about 50% of the uninfected erythrocyte volume. The total volume of the infected erythrocyte remains constant, apart from some reversible shrinkage at stage IV, while the concentration of hemoglobin decreases to about 70% of that in an uninfected erythrocyte.
Authors:
Eric Hanssen; Christian Knoechel; Megan Dearnley; Matthew W A Dixon; Mark Le Gros; Carolyn Larabell; Leann Tilley
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-09-16
Journal Detail:
Title:  Journal of structural biology     Volume:  177     ISSN:  1095-8657     ISO Abbreviation:  J. Struct. Biol.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-20     Completed Date:  2012-06-05     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9011206     Medline TA:  J Struct Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  224-32     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Electron Microscopy Unit, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC 3010, Australia. ehanssen@unimelb.edu.au
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MeSH Terms
Descriptor/Qualifier:
Calibration
Cell Shape
Cell Size
Erythrocytes / metabolism,  parasitology*,  ultrastructure
Germ Cells / physiology,  ultrastructure
Hemoglobins / metabolism*
Humans
Microscopy
Microscopy, Electron, Transmission
Plasmodium falciparum / physiology*,  ultrastructure
Reproduction, Asexual
Serum Albumin, Bovine / chemistry
Single-Cell Analysis
Spores, Protozoan / ultrastructure
Tomography, X-Ray
X-Ray Absorption Spectroscopy
Grant Support
ID/Acronym/Agency:
P41 RR019664/RR/NCRR NIH HHS; RR019664/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Hemoglobins; 0/Serum Albumin, Bovine
Comments/Corrections

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