Document Detail


Sodium thiosulphate impairs the cytotoxic effects of cisplatin on FADU cells in culture.
MedLine Citation:
PMID:  16527011     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: To study the effect of cisplatin (CDDP) on FADU human squamous cell carcinoma cells and to determine if sodium thiosulphate (STS), an agent protective against CDDP ototoxicity, affects the tumoricidal activity of CDDP. METHOD: FADU tumour cells were grown in culture. Cells were exposed to varying concentrations of CDDP with and without concomitant STS, and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assays were then performed to determine the effect of treatment on FADU cell growth. RESULTS: Dose-response curves were generated for the FADU cells exposed to CDDP with and without concomitant STS. Concomitant STS was found to inhibit the tumoricidal activity of CDDP in vitro. CONCLUSIONS: Simultaneous administration of STS to in vitro culture of FADU tumor cells leads to inhibition of CDDP's tumoricidal activity.
Authors:
Norbert R Viallet; Brian B Blakley; Asher Begleiter; Marsha K Leith
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of otolaryngology     Volume:  35     ISSN:  0381-6605     ISO Abbreviation:  J Otolaryngol     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-03-10     Completed Date:  2006-06-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7610513     Medline TA:  J Otolaryngol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  19-21     Citation Subset:  IM    
Affiliation:
Department of Otolaryngology, University of Manitoba, Winnipeg, Manitoba, Canada.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / adverse effects*
Antioxidants / pharmacology*
Carcinoma, Squamous Cell / drug therapy
Cell Line, Tumor
Cisplatin / adverse effects*
Dose-Response Relationship, Drug
Head and Neck Neoplasms / drug therapy
Humans
Thiosulfates / pharmacology*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Antioxidants; 0/Thiosulfates; 15663-27-1/Cisplatin; 7772-98-7/sodium thiosulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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