Document Detail


Sodium sulfite is a potential hypoxia inducer that mimics hypoxic stress in Caenorhabditis elegans.
MedLine Citation:
PMID:  21057967     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Physical and chemical hypoxia have been widely used in the study of hypoxic injury; however, both of these hypoxia models have their own limitations. Physical hypoxia is usually difficult to control and maintain. Chemical hypoxia, which is usually induced by chemical hypoxia-mimicking agents, such as CoCl(2), may result in heavy metal toxicity or impose security threats. To develop a more suitable hypoxia model, we focused on sodium sulfite (Na(2)SO(3)) and evaluated its ability to remove dissolved oxygen in aqueous solutions. Our results showed that sodium sulfite successfully induced hypoxic conditions. The degree of hypoxia and the guarantee period of the sodium sulfite solution could be easily controlled by the concentration of soluble sodium sulfite. In addition, we used sodium sulfite to create a hypoxia model in Caenorhabditis elegans. Similar to physical hypoxia, the sodium sulfite solutions induced hypoxia-related death in the worms and led to morphologic cell defects and C. elegans hypoxia inducible factor 1 stabilization. Taken together, our data show that sodium sulfite is a potential hypoxia inducer that mimics hypoxic stress in C. elegans.
Authors:
Bin Jiang; Changhong Ren; Yuan Li; Yiming Lu; Weiguang Li; Yonghong Wu; Yan Gao; Peter J Ratcliffe; Huqi Liu; Chenggang Zhang
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Publication Detail:
Type:  Journal Article     Date:  2010-10-30
Journal Detail:
Title:  Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry     Volume:  16     ISSN:  1432-1327     ISO Abbreviation:  J. Biol. Inorg. Chem.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9616326     Medline TA:  J Biol Inorg Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  267-74     Citation Subset:  IM    
Affiliation:
Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Taiping Road 27, Beijing, 100850, China.
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